Abstract: AIM: To investigate the gene mutation in self-doubt related to drug-induced hearing loss patients of non-syndromic sensorineural hearing loss and identify the molecular etiopathogenisis.METHODS: Peripheral blood samples were obtained from 35 cases self-doubt hearing loss of patients collected by out-patient clinic. Their genomic DNA was extracted from peripheral blood by extraction kits to undergo polymerase chain reaction, Gene microarray and sequencing so as to detect the mutations of mitochondrial 12S rRNA gene.RESULTS: In 35 patients, one mutation was found out in mtDNA 12S rRNA gene C1494T (2.86%), the other were wild type or other DNA mutations.CONCLUSION: Only a minority of drug-induced hearing loss associated with Mitochondrial DNA mutations amongst clinical self-doubt patients. The other deafness genes test simultaneously would be better. And gene microarray is one of a new and good method suggested.

Key words: Gene microarray, Drug-induced hearing loss, Self-doubt, 12S rRNA, C1494T