Intervention activity of lychee seed saponin against rats with senile dementia and its mechinisms

Abstract

Abstract: AIM: To investigate the activity and mechanisms of lychee seed saponin against rats with senile dementia (AD). METHODS: The 50 AD rats were randomly divided into control group, AD model group, low-dose lychee seed saponin group, medium-dose lychee seed saponin group, and high-dose lychee seed saponin group, respectively. Morris water maze examination was used to detect learning and memory ability. The protein expression was detected by Western blot analysis. The level of reactive oxygen species (ROS) was determined by flow cytometer analysis.RESULTS: The escape latency of rats was significantly increased in AD rats (P<0.01),and the numbers of rats crossing platform was significantly decreased in AD rats (P<0.01). The learning and memory ability were significantly developped by lychee seed saponin treatment. The protein expression of Aβ was significant higher in AD rats than that in normal control. The protein expression of Aβ was decreased in a dose-dependent manner by lychee seed saponin treatment in AD rats. The level of ROS in control group, AD model group, low-dose lychee seed saponin group, medium-dose lychee seed saponin group, and high-dose lychee seed saponin group was 6.12±0.61, 9.54±1.42, 8.33±1.26, 7.68±1.14, and 7.23±0.73, respectively. The level of ROS was significant higher in AD rats than that in normal control. The level of ROS was decreased in a dose-dependent manner by lychee seed saponin treatment in AD rats. CONCLUSION: Lychee seed saponin showed the potent activity against AD rats. The decreasing of Aβ and ROS production may play a key role.

Key words: Senile dementia, Lychee seed saponin, β-amyloid protein(Aβ);, Reactive oxygen species

CLC Number:

R965.2

LI Juan, WANG Hai-tao. Intervention activity of lychee seed saponin against rats with senile dementia and its mechinisms[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(10): 1098-1102.

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