Effects of N-n-butyl haloperidol iodide on the proliferation of intima and vascular muscle cells of rabbit carotid artery after balloon injury

Abstract

Abstract: AIM: To investigate the effects of N-n-butyl haloperidol iodide (F₂) on the proliferation of intima and vascular smooth muscle cells (VSMCs) of the rabbit carotid artery after balloon injury. METHODS: 30 New Zealand rabbits were randomly divided into Sham group, Model group, F₂ high-dose treatment group (2 mg/kg), F₂ medium-dose treatment group (1 mg/kg), F₂ low-dose treatment group (0.5 mg/kg). After 7 days, experimental segments of arteries were harversted and subsequently progressed routine pathological sections, hematoxylin-eosin staining, PCNA expression was measured by immunohistochemical staining. RESULTS: Compared with the sham group, the intimal thickness, intimal area, intimal/medial thickness and intima-to-media area ratio of the Model group significantly increase (P<0.01). F₂ caused a dose-dependent reduction in these indicators. F₂ dose-dependently decrease the PCNA positive rate of VSMCs in the vascular wall as compared with Model group (P<0.01). CONCLUSION: F₂ inhibits the proliferation of intima and VSMCs of rabbit carotid artery after balloon injury.

Key words: N-n-butyl haloperidol iodide, Mechanical injury, Intima, Vascular smooth muscle cells, Proliferation

CLC Number:

R965.2

HUANG Zhan-qin, JIANG Hong-yan, LI Jin-yu, LIU Xing-pin, ZHENG Yan-shan, GAO Fen-fei, CHEN Yi-cun, SHI Gang-gang. Effects of N-n-butyl haloperidol iodide on the proliferation of intima and vascular muscle cells of rabbit carotid artery after balloon injury[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(10): 1112-1117.

References

[1] Togni M, Windecker S, Cocchia R, et al.Sirolimus-eluting stents associated with paradoxic coronary vasoconstriction[J]. J Am Coll Cardio, 2005, 46(2): 231-236.
[2] Pasterkamp G, de Kleijn DP, Borst C. Arterial remodeling in therosclerosis, restenosis and after alteration of blood flow: potential mechanisms and clinical implications[J]. Cardiovasc Res, 2000, 45(4): 843-852.
[3] Muto A, Fitzgerald TN, Pimiento JM, et al.Smooth muscle cell signal transduction: implications of vascular biology for vascular surgeons[J]. J Vasc Surg, 2007, 45(6S): 15-24.
[4] House SJ, Potier M, Bisaillon J, et al.The non-excitable smooth muscle: calcium signaling and phenotypic switching during vascular disease[J]. Pflugers Arch, 2008, 456(5): 769-785.
[5] 邓水秀, 曾泗宇, 任俊芳, 等. 降钙素基因相关肽对大鼠血管平滑肌细胞CDK2和Cyclin E的影响[J]. 中国临床药理学与治疗学, 2011, 16(3): 249-253.
[6] 邓少雄, 郭南鸥, 郑渊, 等. 替罗非班对急性ST段抬高型心肌梗死并糖尿病患者急诊经皮冠状动脉介入治疗术后血清MMP-9水平的影响及临床意义[J]. 中国临床药理学与治疗学, 2011, 16(7): 807-811.
[7] Khachigian LM.Early growth response-1 in cardiovascular pathobiology[J]. Circ Res, 2006, 98(2): 186-191.
[8] Huang Z, Shi G, Gao F, et al.Effects of N-n-butyl haloperidol iodide on L-type calcium channels and intracellular free calium in rat ventricular myocytes[J]. Biochem Cell Biol, 2007, 85(2): 182-188.
[9] Huang Z, Li H, Guo F, et al.Egr-1, the potential target of calcium channel blockers in cardioprotection with ischemia/reperfusion injury in rats[J]. Cell Physiol Biochem, 2009, 24(1/2): 17-24.
[10] Morishita R, Gibbons GH, Ellison KE, et al.Intimal hyperplasia after vascular injury is inhibited by antisense cdk 2 kinase oligonucleotides[J]. J Clin Invest, 1994, 93(4): 1458-1464.
[11] Zheng B, Han M, Bernier M, et al.Krüppel-like factor 4 inhibits proliferation by platelet-derived growth factor receptor β-mediated, not by retinoic acid receptor α-mediated, phosphatidylinositol 3-kinase and ERK signaling in vascular smooth muscle cells[J]. J Biol Chem, 2009, 284(34): 22773-22785.
[12] Indolfi C, Torella D, Coppola C, et al.Rat carotid artery dilation by PTCA balloon catheterinduces neointima formation in presence of IEL rupture[J]. Am J Physiol Heart Circ, 2002, 283(2): H760-H767.
[13] 韩健, 郭富强. 三七通舒胶囊对颅内外动脉狭窄支架成形术后血清IL-1β和IL-8表达的影响[J]. 中国临床药理学与治疗学, 2011, 16(8): 925-928.
[14] Wang L, Zheng J, Bai X, et al.ADAMTS-7 mediates vascular smooth muscle cell migration and neointima formation in balloon-injured rat arteries[J]. Circ Res, 2009, 104(5): 688-698.
[15] Owens GK.Regulation of differentiation of vascular smooth muscle cells[J]. Physiol Rev, 1995, 75(3): 487-517.
[16] Regan CP, Adam PJ, Madsen CS, et al.Molecular mechanisms of decreased smooth muscle differentiation marker expression after vascular injury[J]. J Clin Invest, 2000, 106(9): 1139-1147.