Abstract: AIM: To optimize perfusate composition of in situ perfused rat intestine-liver model and achieve the best perfusate formula. METHODS: The perfusion conditions were optimized, respectively. The Krebs-Ringer NaHCO₃ solution with bovine serum albumin and mannitol and the Krebs-Ringer NaHCO₃ solution with bovine serum albumin and dextran two T40 were contrasted for finding out the best perfusate medium. Then bovine serum albumin content in the optimal perfusion medium was optimized. RESULTS: K-R solution with bovine serum albumin and dextran T40 group was better than K-R solution with bovine serum albumin and mannitol group; 5% bovine serum albumin in perfusate is a more economic and ideal proportions. CONCLUSION: 5% bovine serum albumin in perfusate is a more economic and ideal proportions.

Key words: In situ perfused rat intestine-liver model, Dextran T40, Mannitol, Bovine serum albumin