Heat shock protein 70 peptide inhibits Th17 cells in collagen-induced arthritis mice

Abstract

Abstract: AIM: The effect of heat shock protein 70 (HSP70) peptide on Th17 cells was studied in collagen-induced arthritis(CIA) mice model and the mechanism of its inhibitory effect on inflammation was also investigated. METHODS: DBA/1 mice were randomly divided into four groups including HSP70 peptide group, negative control group, PPD treated group and positive control group. Arthritis was induced by immunizing bovine typeⅡcollage, except negative control group. Pathology was evaluated by paw swelling and histological changes. The number of Th17 cells in peripheral lymph nodes was assayed by flow cytometry, and Th17 cell-associated regulatory factors were measured by real-time quantitative PCR. RESULTS: The number of Th17 cells in HSP70 peptide treated group was significantly lower than that of positive control group and PPD treated group. Expression levels of IL-6, IL-23, TGF-β₁ and orphan nuclear receptor(RORγt) in HSP70 peptide group were markedly reduced than those of positive group and PPD group. CONCLUSION: HSP70 peptide could play a protective role on CIA mice through decreasing Th17 cell regulating factors and inhibiting the differentiation of Th17 cells.

Key words: Heat shock protein 70, T helper 17 cells, Collagen-induced arthritis mice

CLC Number:

R392

GUO Hong-yan^1,2, YUAN Hui-hui¹, ZHANG Xu-long¹, ZHAO Wen-ming¹. Heat shock protein 70 peptide inhibits Th17 cells in collagen-induced arthritis mice[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(4): 392-397.

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