Effects of exogenous endothelium-dependent hyperpolarizing factor on cerebral ischemia reperfusion injury

Abstract

Abstract: AIM: Hydrogen sulfide (H₂S) is one of the supposed endothelium-dependent hyperpolarizing factor (EDHF), the protective effects of exogenous H₂S, namely exogenous supposed EDHF on cerebral ischemia reperfusion (I/R) injury was observed in present study. METHODS: Sodium hydrosulfide (NaHS) was taken as a donor of H₂S. Focal cerebral I/R injury was caused by occluding the right middle cerebral artery (MCAO) in rats. The numerical data, which were from infarct volume, neurological deficit, brain water content, malondialdehyde (MDA) level and lactate dehydrogenase (LDH) activities in serum, were evaluated. HE staining was used to observe brain pathologic changes. RESULTS: 0.195, 0.390, 0.780 mg/kg NaHS markedly ameliorated the neurological deficit, decreased the percentage of infarct volume, reduced the brain water content, and dramatically inhibited the cerebral I/R injury-induced increases of LDH activity and MDA content in serum, improved brain pathologic changes in different degrees. CONCLUSION: NaHS significantly improved the cerebral I/R injury in rats, this suggests exogenous supposed EDHF (H₂S) has a significant protective effect against cerebral I/R injury.

Key words: Endothelium-dependent hyperpolarizing factor, Hydrogen sulfide, Sodium hydrosulfide, Cerebral ischemia reperfusion injury

CLC Number:

R965.2

JIAO Chuan-an, SONG Biao, CHEN Zhi-wu. Effects of exogenous endothelium-dependent hyperpolarizing factor on cerebral ischemia reperfusion injury[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(6): 648-653.

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