Abstract: AIM: To observe the effect of rhubarb on intestinal bacterial translocation in rats with hyperlipoidemia and acute pancreatitis. METHODS: 60 SD rats were randomly divided into 5 groups: control group, AP group, HLAP group, AP treatment group, HLAP treatment group. We established rats model of AP and HLAP, rats in treatment groups were treated by rhubarb. The levels of serum amylase and triglyceride, intesital permeability, mucosa structure of small intestine were checked. Portal vein blood, pancreas and mesenteric lymph node were collected for 16s rRNA gene and culture. RESULTS: The levels of serum amylase in HLAP group and HLAP treatment group were lower than in AP group and AP reatment group 24h after the operation (P<0.05). The serum amylase 6 days after the operation in both treatment groups were lower than those 24 h after the operation (P<0.01). The levels of serum triglyceride were significant high in HLAP group and HLAP treatment group compared to the other 3 groups 24 h after the operation (P<0.01), the levels in all 5 groups fell 6 days after the operation and it was most obvious in HLAP treatment group. L/M was higher in HLAP group than in AP group 3d after the operation (P<0.05). L/M in AP treatment group was lower than in AP group (P<0.05), and L/M in HLAP treatment group was lower than in HLAP group (P<0.01). In AP group and HLAP group, villi of small intestine was engorged and destroyed, proper layer was infiltrated by inflammatory cells ,mucosa damage was mild in both treatment groups. Positive rate of culture and 16s rRNA gene in HLAP group were significantly higher than AP group (P<0.01). Positive rate in AP treatment group and HLAP treatment group were lower than AP group and HLAP group respectively (P<0.01).CONCLUSION: Compared to AP ,the intestinal bacterial translocation in HLAP is severe. Rhubarb can treat hyperlipoidemia in HLAP, and it has obvious effect on intestinal bacterial translocation.

Key words: Pancreatitis, Hyperlipoidemia, Rhubarb, Bacterial translocation