Effects of crocetin on proliferation and collagen synthesis of hepatic stellate cell

Abstract

Abstract: AIM: To investigate the effects of crocetin on proliferation, collagen synthesis in hepatic stellate cell (HSC) stimulated by acetaldehyde and its partial mechanism.METHODS: HSC stimulated by acetaldehyde was incubated with different doses of crocetin(10^-8,10^-7,10^-6 mol/L), cell proliferation was analyzed by MTT assay, apoptosis ratio was analyzed by flow cytometry(FCM), synthesis of collagen was observed by hydroxyproline concentration measurement. The ERK1/2, Bax, Bcl-2 were tested by Western blotting. Matrix metalloproteinase(MMP-2),the tissue inhibitor metalloproteinase (TIMP-1) mRNA, collagen mRNA were examined by RT-PCR.RESULTS: Within a concentration coverage, crocetin inhibited proliferation of HSC induced by acetaldehyde. The apoptosis rate of cells in crocetin treated groups increased with concentration compared with acetaldehyde group. Crocetin decreased the levels of ERK1/2 and Bcl-2 expression, but increased Bax protein expression. Crocetin could markedly suppressed the up-regulation of CollagenⅠmRNA, Collagen ⅢmRNA, TIMP-1 mRNA, while increased MMP-2 mRNA expression.CONCLUSION: Crocetin inhibited HSC proliferation and collagen synthesis stimulated by acetaldehyde, which related to the expression of ERK1/2 and the regulation of extracellular matrix.

Key words: Crocetin, Liver fibrosis, Acetaldehyde, Collagen

CLC Number:

R965.2

ZHU Yan-hong, CHEN Zhen, QIAN Zhi-yu, HU Hui, CHENG Wen-yuan. Effects of crocetin on proliferation and collagen synthesis of hepatic stellate cell[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(8): 841-846.

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