nNOS is required for enhancing proliferation of NSCs induced by OGD

Abstract

Abstract: AIM: To explore the mechanism of ischemia-induced neurogenesis in vitro. METHODS: Monolayer-cultured neural stem cells (NSCs) or neurons (before co-culture) were exposed to oxygen and glucose deprivation (OGD) for 2 h or 2.5 h. Cell proliferation was assessed by BrdU incorporation. We also performed RT-PCR, western blot, immunofluorescence, NOS activity assay and NOx content measurement to detect alteration of neuronal nitric oxide synthase (nNOS) in NSCs and neurons. RESULTS: That NSCs exposed to OGD alone or co-cultured with the OGD-injured neurons displayed a remarkably active proliferation rate. It was demonstrated that OGD exposure up-regulated nNOS in NSCs and down-regulated nNOS in neurons. nNOS knockout in NSCs or neurons( for co-culture experiment) reversed the enhanced proliferation rate of NSCs induced by OGD.CONCLUSION: nNOS alterations both in NSCs and neurons accounts for the OGD-induced NSCs proliferation.

Key words: neuronal nitric oxide synthase, neural stem cells, OGD, proliferation

CLC Number:

R965.2

QIAN Xiao-dan, LUO Chun-xia, ZHU Dong-ya. nNOS is required for enhancing proliferation of NSCs induced by OGD[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(10): 1099-1106.

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