Clinical application of monitoring thiopurine methyltransferase gene polymorphism and 6-thioguanine nucleotides concentration in red blood cells on inflammatory bowel disease patients with azathioprine treatment

Abstract

Abstract: AIM: To investigate the clinical application of monitoring TPMT gene polymorphism and 6-TGNs concentration on inflammatory bowel disease (IBD) patients with azathioprine treatment, so as to implementing individual dosage regimen.METHODS: Patients diagnosed with IBD were met the inclusion criteria, the TPMT*3 genotyping assay was based on polymerase chain reaction and Pyrosequencing for TPMT exon 7 (G460A) and TPMT exon 10 (A719G). The concentrations of 6-TGNs in red blood cells were measured by high-performance liquid chromatography.RESULTS: For 15 patients, TPMT^*3 genotyping were wild type and no mutation was found. The initial concentrations of 6-TGNs were 147.31-875.26 pmol per 8×10⁸ RBC and significant individual differences were obvious for patients with same dose azathioprine.CONCLUSION: TPMT genotyping is useful to predict the myelosuppression, while 6-TGNs monitoring is helpful to adjusting dose of azathioprine. Application of combining TPMP genotyping and 6-TGNs monitor may offer a basis for individualized and safe medication for IBD patients.

Key words: inflammatory bowel disease, thiopurine methyltransferase gene polymorphism, 6-TGNs concentration in red blood cells, clinical application

CLC Number:

R969

XIA Quan, HUANG Yan, WANG Yan-yan, XU Du-juan, HU Nai-zhong, HU Jing, MEI Qiao, CHEN Fei-hu. Clinical application of monitoring thiopurine methyltransferase gene polymorphism and 6-thioguanine nucleotides concentration in red blood cells on inflammatory bowel disease patients with azathioprine treatment[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(3): 302-308.

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