The detection of plasmid mediated quinolone resistance genes in carbapenem-resistant klebsiella-pneumoniae clinical isolates

Abstract

Abstract: AIM: To investigate distribution of plasmid mediated quinolone resistance(PMQR) genes in carbapenem-resistant klebsiella-pneumoniae(CRKP) clinical isolates.METHODS: 29 CRKPs were collected , PCR amplifications were performed and the products were sequenced to determine the genetypes.RESULTS: The detection rate of PMQR genes was 48.3%(14/29),5 strains carring qnrB gene, including qnrB2 1 strain,qnrB4 3 strain,qnrB10 1 strain; 5 strains carring qnrS1 gene; 1 strain both carring qnrS1 and qnrB4; 3 strains carring aac(6')-Ib-cr gene. All the 14 strains were positive for PMQR genes harbored β-lactamase genes and 8 strains also harbored carbapenemase genes, accounting for 57.1%(8/14),blaKPC-2(4/14)and blaNDM-1(3/14)were the main genotypes of carbapenemase genes.CONCLUSION: PMQR genes are common among the clinical isolates of CRKP, predominantly qnr genotype,and the carring rate of carbapenemase genes is high in qnr-positive isolates,all which are multiple drug-resistant strains.

Key words: plasmid mediated quinolone resistance gene, drug resistance, carbapenem-resistance, klebsiella-pneumoniae

CLC Number:

R446.5

HANG Ya-ping, NING Chang-xiu, WANG Hong, ZHONG Qiao-shi, HU Xiao-yan, ZHANG Bai-ling, ZHANG Nan, HU Long-hua. The detection of plasmid mediated quinolone resistance genes in carbapenem-resistant klebsiella-pneumoniae clinical isolates[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(5): 517-521.

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