Protection of losartan on cultured rats hippocampal neurons odels of hypoglycemia anoxia injury in vitro

Abstract

Abstract: AIM: To study the effects and possible mechanisms of losartan on cultured rats hippocampal neurons models of hypoglycemia anoxia injury in vitro. METHODS: The hippocampal neurons were cultured in vitro,and then cells were randomly divided into the hypoxic ischemic injury group,losartan low-dose group,losartan high-dose group.The cell model of hypoxic ischemic was established by oxygen-glucose deprivation mimicking cerebral hypoxic ischemic injury.Neuron apoptosis in hippocampus tissue were determined with Tunel staining.The expression levels of active-caspase-3 protein were observed by Western blot analysis.RESULTS: 12 h deprivation of oxygen-glucose induced cell apoptosis of hippocampal neurons. Cell apoptosis and expression of active caspase-3 were significantly decreased in pre-treatment losartan group.CONCLUSION: Losartan had protective effects on cultured hypoxic ischemic hippocampal neurons of rats.The mechanisms may be related with resisting apoptosis and caspase related pathway.

Key words: losartan, hypoglycemia anoxia, apoptosis

CLC Number:

R965.2

CHEN Jiang-ying, YAN Zhen-wen, ZHANG Su-ping, YIN Yi-chen. Protection of losartan on cultured rats hippocampal neurons odels of hypoglycemia anoxia injury in vitro[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(9): 984-987.

References 10

[1]	张凤云,赵炎,吴玉林. 蛋白激酶C在脑缺血再灌注损伤中的作用[J]. 中国临床药理学与治疗学,2013,18(4):461-468.
[2]	陈江瑛,闫振文,张付生,等. 瘦素对血管性痴呆大鼠认知功能的改善保护作用[J]. 中华行为医学和脑科学杂志,2010, 19(7): 621-623.
[3]	Baron JC, Yamauchi H, Fujioka M, et al.Selective neuronal loss in ischemic stroke and cerebrovascular disease[J]. J Cereb Blood Flow Metab,2014,34(1):2-18.
[4]	Mogi M, Horiuchi M, Effects of angiotensin II receptor blockers on dementia[J]. Hypertens Res,2009,32(9):738-740.
[5]	Albiston AL, Fernando RN, Yeatman HR, et al.Gene knockout of insulin-regulated aminopeptidase:loss of the specific binding site for angiotensin IV and age-related deficit in spatial memory[J]. Neurobiol Learn Mem,2010,93(1):19-30.
[6]	Washida K, Ihara M, Nishio K, et al.Nonhypotensive dose of telmisartan attenuates cognitive impairment partially due to peroxisome proliferator-activated receptor-gamma activation in mice with chronic cerebral hypoperfusion[J]. Stroke,2010, 41(8):1798-1806.
[7]	Wright JW, Harding JW.The brain RAS and Alzheimer's disease[J]. Exp Neurol,2010,223(2):326-333.
[8]	Marc Y, Llorens-Cortes C.The role of the brain renin-angiotensin system in hypertension: Implications for new treatment[J]. Progress in Neurobiology,2011,95(2):89-103.
[9]	Faure S, Chapot R, Tallet D, et al.Cerebroprotective effect of angiotensin IV in experimental ischemic stroke in the rat mediated by AT(4) receptors[J]. J Physiol Pharmacol,2006,57(3):329-342.
[10]	Moretti A, Gorini A, Villa RF.Pharmacotherapy and prevention of vascular dementia[J]. CNS & Neurological Disorders-Drug Targets, 2011,10(3):370-390.

[1]	FAN Jie, JIN Yongming, JIANG Xiaolong, JIANG Guohua. Molecular mechanism of lncRNA HOTAIR regulating miR-206 on the proliferation and apoptosis of rheumatoid arthritis synovial cells [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(7): 736-742.
[2]	YANG Xiaojia, JIANG Meng, WU Min, ZHANG Yili, LV Lan, WU Yuanfen, WANG Xinyu, LIU Liquan. Improvement effect of crocin on cognitive impairment of Alzheimer's disease rats through DKK3 regulation of GSK-3 β/β-Catenin pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(5): 489-497.
[3]	ZHANG Xuejiao, LIU Jieting, LI Luxin, CHEN Peijian, DING Minglu, SUN Mengwei, CHU Yanhui, ZHANG Zhen . Effects and mechanism of dapagliflozin on myocardial injury in type 1 diabetes mice [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(3): 257-265.
[4]	HOU Xiaoyu, LENG Yufang, CAO Xuefen, LV Xingjiao, HAN Xiaoxia, Janvier NIBARUTA, LIU Yongqiang. Network analysis and experimental verification of Schisandrin B reduces intestinal ischemia reperfusion injury [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(2): 147-154.
[5]	WANG Jian, SUN Yongdong, ZHOU Xingwei, LIU Lei, CHEN Long, TONG Xingke, ZHU Jiali. Baicalein promotes laryngeal cancer cell death and inhibits invasion via miR-125b-5p/IRF4 axis [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(11): 1209-1218.
[6]	ZHU Ruifang, GUO Dongkai, ZHI Hui, JIANG Yiguo, ZHANG Yueling, QIAN Xiaoping, JI Shiliang. Exploring the mechanism of elemene synergistic bortezomib against multiple myeloma by ROS-NF-κB-p38MAPK pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(11): 1219-1226.
[7]	ZHOU Huan, XU Ming, LI Bo, LIU Chunying, WANG Chun. PI3K/Akt/FOXO3a signaling pathway inducing protective autophagy promotes acquired lung adenocarcinoma resistance remodeling to DDP [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(9): 961-970.
[8]	HUA Haiyan, YAN Jie, QIN Yufen. Experimental study of schisandrin B attenuates acute myocardial ischemia injury in rats by inhibiting cardiomyocyte apoptosis [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(8): 848-856.
[9]	LI Xueheng, LI Long. Research progress on the relationship between melatonin and idiopathic pulmonary fibrosis [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(6): 715-720.
[10]	YANG Liu, LUO Ailin, LI Shiyong. Advance of general anesthetics-induced developing brain injury [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(12): 1381-1390.
[11]	TAN Fenglang, REN Hongli, WANG Hongfei. Effect of Phellodendri amurense extract on hippocampal nerve cells induced by Aβ_1-42 [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(11): 1213-1220.
[12]	PAN Rongrong, ZHI Yinghao, JIN Yongxi, ZHOU Xiahui. Influences of theaflavins on neuronal apoptosis and blood-brain barrier in rats with cerebral hemorrhage by regulating CaMKK2/AMPK signaling pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(11): 1240-1246.
[13]	LI Ying, YAO Wei, WANG Meng, YU Zhihong, GONG Yuanqi, LAN Haibing, QI Xiefei. Experimental study of irisin alleviates house dust mite-induced airway epithelial cells inflammation and apoptosis via the NF-κB and JNK signaling pathways [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(10): 1106-1112.
[14]	LIU Yunfan, DU Yuemei, LIU Xiaoyi, GAO Liping. Effects of lycium barbarum polysaccharides on cis-dichlorodiamineplatinum (II)-induced apoptosis in mouse testis sertoli cells TM4#br# [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2021, 26(6): 624-630.
[15]	WEI Songchang, YANG Hongqi, ZHANG Hongjuan, SHI Xiaoli, REN Kaiwen. Correlation between the expression of RON protein and CXC chemokine receptor 4 protein and abiraterone resistance in patients with castration-resistant prostate cancer [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2021, 26(4): 414-422.