Effects of aspirin on inflammatory responses in PCOS rats

Abstract

Abstract: AIM: To study the effects of aspirin on inflammatory responses in polycystic ovarian syndrome (PCOS) rats. METHODS: Combined administration of human HCG and insulin was used for establishing PCOS rat model, PCOS rat weight, ovarian weight, morphology. The effects of aspirin on the levels of serum TNF-α, IL-6 and insulin and the expression of IL-6 and TNF-α mRNA in ovarian tissue were observed. RESULTS: Aspirin treatment significantly reduced the rat weight and ovary weight in PCOS rats, the ovarian pathological changes, levels of TNF-α, IL-6 and insulin in serum and expressions of IL-6 and TNF-α mRNA in ovarian tissue were obviously reduced compared with the PCOS rats. CONCLUSION: Aspirin therapy has protective effect on PCOS rats and the effect is closely related to its anti-inflammatory effect.

Key words: aspirin, polycystic ovary syndrome, inflammatory responses

CLC Number:

R965.2

LI Wei-hong, LI Bin. Effects of aspirin on inflammatory responses in PCOS rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2015, 20(1): 19-23.

References

[1] Kruszynska A, Slowinska-Srzednicka J, Jeske W, et al. Proinsulin, adiponectin and hsCRP in reproductive age women with polycystic ovary syndrome (PCOS)-the effect of metformin treatment[J]. Endokrynol Pol, 2014, 65(1): 2-10.
[2] 胡卫华, 徐庆阳, 唐静文. 来曲唑与枸橼氯米芬对多囊卵巢综征患者诱导排卵的效果分析[J]. 中国临床药理学与治疗学, 2013, 18(7): 789-793.
[3] Escobar-Morreale HF, Luque-Ramirez M, Gonzalez F. Circulating inflammatory markers in polycystic ovary syndrome: a systematic review and metaanalysis[J]. Fertil Steril, 2011, 95(3): 1048-1058.
[4] Garcia-Garcia FJ, Mullol J, Perez-Gonzalez M, et al. Signal transduction pathways (MAPKs, NF-kappaB, and C/EBP) regulating COX-2 expression in nasal fibroblasts from asthma patients with aspirin intolerance[J]. PLoS One, 2012, 7(12): e51281.
[5] 叶慧君, 江延姣, 李爱萍, 等. 桂枝茯苓胶囊联合达英-35和枸椽酸氯米芬治疗多囊卵巢综合征合并不孕症的临床观察[J]. 中国临床药理学与治疗学, 2012, 17(6): 691-695.
[6] Buettner R, Ascher M, Gabele, E, et al. Olive oil attenuates the cholesterol-induced development of nonalcoholic steatohepatitis despite increased insulin resistance in a rodent model[J]. Horm Metab Res, 2013, 45(11): 795-801.
[7] Carvalho-Filho MA, Ropelle ER, Pauli RJ, et al. Aspirin attenuates insulin resistance in muscle of diet-induced obese rats by inhibiting inducible nitric oxide synthase production and S-nitrosylation of IRbeta/IRS-1 and Akt[J]. Diabetologia, 2009, 52 (11): 2425-2434.
[8] Poretsky L, Clemons J, Bogovich K. Hyperinsulinemia and human chorionic gonadotropin synergistically promote the growth of ovarian follicular cysts in rats[J]. Metabolism, 1992, 41(8): 903-910.
[9] 邓阿黎, 周忠明, 张亚兵, 等. 补肾化痰祛疲中药对PCOS模型大鼠卵巢组织形态学作用的实验研究[J]. 中国药师, 2009, 12(2): 143-145.
[10] Li H, Chen Y, Yan LY, et al. Increased expression of P450scc and CYP17 in development of endogenous hyperandrogenism in a rat model of PCOS[J]. Endocrine, 2012, 43(1): 184-90.
[11] Kelly CCJ, Lyall H, Petrie JR, et al. Low grade chronic inflammation in women with polycystic ovarian syndrome[J]. J Clin Endocrinol Metab, 2001, 86(6): 2453-2455.
[12] Repaci A, Gambineri A, Pasquali R. The role of low-grade inflammation in the polycystic ovary syndrome[J]. Mol Cell Endocrinol, 2011, 335(1): 30-41.
[13] Fulghesu AM, Sanna F, Uda S, et al. IL-6 serum levels and production is related to an altered immune response in polycystic ovary syndrome girls with insulin resistance[J]. Mediators Inflamm, 2011, 2011(1): 389317.
[14] Amato G, Conte M, Mazziotti G, et al. Serum and follicular fluid cytokines in polycystic ovary syndrome during stimulated cycles[J]. Obstet Gynecol, 2003, 101(6): 1177-1182.
[15] Hundal RS, Petersen KF, Mayerson AB, et al. Mechanism by which high-dose aspirin improves glucose metabolism in type 2 diabetes[J]. J Clin Invest, 2002, 109(10): 1321-1326.
[16] Block R, Abdolahi A, Smith B, et al. Effects of low-dose aspirin and fish oil on platelet function and NF-kappaB in adults with diabetes mellitus[J]. Prostaglandins Leukot Essent Fatty Acids, 2013, 89(1): 9-18.
[17] Zhao X, Boenisch O, Yeung M, et al. Critical role of proinflammatory cytokine IL-6 in allograft rejection and tolerance[J]. Am J Transplant, 2012,12(1): 90-101.
[18] Wang WJ, Hao CF, Yi L, et al. Increased prevalence of T helper 17 (Th17) cells in peripheral blood and decidua in unexplained recurrent spontaneous abortion patients[J]. J Reprod Immunol, 2010, 84(2): 164-170.