Abstract: AIM: To observe the effects of Weiliuan (compound recipe,sanleng and Ezhu,without Sanleng or Ezhu)low dose group, middle dose group and high dose group on proliferation and cell cycle of human gastric carcinoma cell (SGC-7901)24 and 48 hours. METHODS: Subculture was carried on by human gastric carcinoma cell line SGC-7901 in RPMI1640 culture solution.MTT assay was used to examine the proliferation of SGC-7901 cells. Flow cytometry was used to detect the cell cycle of SGC-7901. We also treated SGC-7901 cells with three doses of Weiliuan for different times. RESULTS: MTT assay showed that three different prescriptions of Weiliuan inhibited the proliferation of SGC-7901 cell; cell cycle analysis indicated that Weiliuan (compound recipe) and Weiliuan (without Sanleng or Ezhu) arrested SGC-7901 at S phase,and Weiliuan (Sanleng and Ezhu) can arrest cell cycle of G₀/G₁ phase, which were on time and dose dependent manner. CONCLUSION: All three of the prescriptions inhibit proliferation of SGC-7901 cells,and arrest cell cycle of S or G₀/G₁ phase.The effect of Weiliuan (compound recipe) and Weiliuan (without Sanleng or Ezhu) are better than Weiliuan (sanleng and Ezhu).

Key words: Weiliuan, gastric carcinoma cell, cell proliferation, cell cycle