Abstract: AIM: To observe the protective function of anti-platelet thrombolysin(APT) on cerebral focal ischemia reperfusion injury in rats and its possible mechanisms.METHODS: SD rats were randomly divided into six groups:sham group,model group,positive control group,APT 0.02,0.01,0.005 mg/kg group. The chronic cerebral ischemia models were made by permanent bilateral occlusion of common carotid anteries. The water contents were measured by dry/wet weight method. The pathology changes of cerebral tissue were observed using light microscope .The focal ischemia-reperfusion injury model was induced by ligation of middle cerebral artery occlusion(MCAO), neurobehavioral score was evaluated in each group, the expressions of TLR4,JNK,Bax were detected by ELISA and immunohistochemistry.RESULTS: Compared with the model Group, the symptoms of neurological deficits were obviously relieved in APT(0.02,0.01 mg/kg)group;the water contents in brain tissue were decreased;the pathological changes were improved and the protein expressions of TLR4,JNK,Bax were reduced.CONCLUSION: APT has protective effect on focal cerebral ischemia/reperfusion injury in rats,which maybe related to the inhibition of apoptosis through restraining the expression of TLR4/JNK/Bax signal pathway.

Key words: anti-platelet thrombolysin, neurological score, TLR4, JNK, Bax