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Chinese Journal of Clinical Pharmacology and Therapeutics ›› 2017, Vol. 22 ›› Issue (6): 622-626.

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Mechanism of human urotensin II on myocardial ischemia-reperfusion injury in rats

WANG Rongjun 1, DING Bo 2   

  1. 1 Medical Branch, Hefei Technology College, Hefei 238000, Anhui, China; 2 Guangzhou Annuanjia Medical Instrument Limited Company, Guangzhou 510000, Guangdong, China
  • Received:2017-04-01 Revised:2017-04-26 Online:2017-06-26 Published:2017-06-26

Abstract:

AIM: To investigate the mechanism of human urotensin II (hUII) against myocardial ischemia-reperfusion injury in rats. METHODS: On the ligation of anterior decending branch of left coronery artery model, changes of electrocardiogram (ECG) were observed, myocardial infarction volume and inducible nitric oxide synthesis (iNOS) mRNA in myocardial tissue were measured. RESULTS:0.47, 1.4 and 4.2 μg/kg hUⅡ obviously inhibited ECG ST segment elevation and myocardial infarction volume in ischemia-reperfusion injured rats; 4.2 μg/kg hUII significantly improved changes of ultrastructure in myocardial cells and increased the expression of iNOS mRNA in myocardium in rats subjected to myocardial ischemia-reperfusion; UT receptor antagonist urantide (10 nmol/kg) significantly antagonized inhibitory effects of hUII 1.4 and 4.2 μg/kg for ECG ST segment elevation and myocardial infarction. CONCLUSION: Mechanism of hUII against myocardial ischemic injury in rats is related to activation of UT receptor and promotion of nitric oxide production.

Key words: human urotensin II, urantide, myocardial ischemia-reperfusion, inducible nitric oxide synthesis, antagonize

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