Inhibitory effect of HLX1 on proliferation and invasion of acute myeloid leukemia cell

Abstract

Abstract:

AIM: To explore the effect of HLX1 on the proliferation and invasion ability in acute myeloid leukemia cell U937. METHODS: The HLX1 mRNA-targeting siRNA was transfected to U937 cells, the mRNA and protein expression of HLX1 were assessed by RT-PCR and Western blot, respectively. MTT assay and Transwell chamber assay were used to observe the proliferation and invasion ability of U937 cell after being transfected with HLX1 mRNA-targeting siRNA. RESULTS:Compared with the control group, HLX1 siRNA transfection reduced the level of HLX1 mRNA in U937 cells after 24 h, the inhibition rate was (70.0±2.7)% (P<0.01)； HLX1 siRNA transfection also reduced the expression of HLX1 protein in U937 cells after 48 h, the inhibition rate was (81.0±3.1)% (P<0.01); HLX1 transfection group cell proliferation was inhibited significantly (P<0.01); The invasion cells of HLX1 siRNA(22.0±2.3) were significantly lower than that of control group(66.0±5.0)(P<0.01), the expression of PAK1 protein was decreased in HLX1 transfection group(P<0.01). CONCLUSION: SiRNA can significantly inhibit the proliferation and invasion of acute myeloid leukemia cell by down-regulating the expression of HLX1.

Key words: acute myeloid leukemia cell, U937, HLX1, RNA interference, invasion

CLC Number:

R965.2
R552

SHEN Jian, LIN Yingchao, ZHU Xiayin. Inhibitory effect of HLX1 on proliferation and invasion of acute myeloid leukemia cell[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(6): 627-632.

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