Effects of emodin on T helper cell subsets of experimental autoimmune thyroiditis mice

Abstract

Abstract:

AIM: To investigate the protective effect of emodin on thyroid in experimental autoimmune thyroiditis (EAT) mice, and its mechanism. METHODS: EAT animal model was induced by iodine on non-obese diabetic (NOD) mice. Four weeks after modeling, experimental groups were treated with emodin with different doses. The TgAb level in plasma and thyroid inflammation were detected eight weeks after modeling to evaluate the protective effect of emodin on EAT mice. T cell subset in peripheral blood and spleen were detected by flow cytometry. IL-4 and IFN-γ were measured by ELISA method. RESULTS:（1）The histopathological study revealed that inflammatory infiltration in thyroid was significant reduced compared with control group (P<0.01).（2）Levels of plasma TgAb were significant increased in each group after modeling, and the increasing amplitudes in emodin treated groups were significantly less than that in model group (P<0.01). （3）After modeling, levels of plasma IL-4, IFN-γ and the cell frequencies of CD+3 CD+ 4IL-4+,CD+ 3CD+4IFN-γ+ ,CD+3CD+8IL-4+ and CD+ 3CD+8IFN-γ+ T cells in peripheral blood monocyte and splenic lymphocyte were significant increased in each group compared with the control group (P<0.01). While the increasing amplitudes in emodin treated groups were less than that in model group. CONCLUSION: The EAT model is viable through an excessive iodine-induced method in NOD mice, and emodin shows a certain inhibitory effect on autoimmune response in EAT mice. This inhibitory effect could be performed by inhibiting the secretion of IFN-γ and IL-4 in CD+ 4 and CD+ 8T cells, and thereby inhibiting the autoimmune response in EAT mice.

Key words: experimental autoimmune thyroiditis (EAT), emodin, Th cell, NOD mice

CLC Number:

R965.2

WU Keren, YE Zhipeng, JIN Fa, FANG Rong, XU Tao, LI Ning. Effects of emodin on T helper cell subsets of experimental autoimmune thyroiditis mice[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(6): 633-639.

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