Effects of procyanidins on TLR4/NF-κB signaling pathway in rats with acute gouty arthritis

doi:10.12092/j.issn.1009-2501.2018.01.008

Abstract

Abstract:

AIM: To observe the effect of procyanidins on acute gouty arthritis SD rats and to explore its possible mechanism. METHODS:SD rats were randomly divided into six groups: the normal group, the model group, low dose group (30 mg/kg）, middle dose group (60 mg/kg), high dose group (90 mg/kg) and colchicine positive control group (0.3 mg/kg). Administration group and control group were given the corresponding dose of drugs by intragastric administration, the normal and model groups were given the same volume of saline. Model of acute gouty arthritis was established by injecting sodium urate into ankle joint cavity. At the fixed time point, joint swelling and mechanical pain threshold were determined, the gait was observed. The levels of IL-1beta, IL-6 and TNF-alfa in serum were measured by ELISA; the protein expression of TLR4 and NF-kB in synovial tissue was measured by Western blot. RESULTS: Procyanidins can relieve swelling of the ankle, improve gait changes and pain changes in rats. At the same time, procyanidins can inhibit the expression of TLR4 and NF-kB protein in SD rats with acute gouty arthritis, down-regulate the levels of IL-1beta, IL-6 and TNF-alfa in serum. CONCLUSION: Proanthocyanidins exhibit a certain effect on SD rats with acute gouty arthritis. The mechanism may be related to the inhibition of TLR4/NF-kB signaling pathway, thereby inhibiting IL-1beta and other inflammatory factors.

Key words: proanthocyanidins, acute gouty arthritis, inflammatory factor

CLC Number:

R965.2

LUO Fei, MEI Yan. Effects of procyanidins on TLR4/NF-κB signaling pathway in rats with acute gouty arthritis[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(1): 41-46.

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