Effects of endoplasmic reticulum associated degradation protein Derlin-1 on the proliferation of human colon cancer cells via suppressing ERK pathway

doi:10.12092/j.issn.1009-2501.2018.12.006

Abstract

Abstract:

AIM: To observe the effects of endoplasmic reticulum associated degradation protein Derlin-1 on the apoptosis of human colon cancer cells (SW480) and its possible mechanism. METHODS: The qualified recombinant plasmid sh-Derlin-1-pGPU6/Neo was transfected into SW480 cells with Derlin-1 knocking down. The expression level of Derlin-1 was detected at both mRNA and protein levels by real-time PCR and Western blot. CCK8 assay and flow cytometry were performed to detect the proliferation and apoptosis of SW480 cells. We examined the expression of apoptosis associated proteins (Bcl-2 and Bax) and ERK phosphorylation followed by Derlin-1 low-expression by Western blot. RESULTS: The Derlin-1 knocking down plasmid was constructed successfully. SW480 cells with Derlin-1 knocking down had been established successfully. Compared with control group, the proliferation rates of sh-Derlin-1 SW480 cells were significantly decreased, while the apoptotic rates of sh-Derlin-1 SW480 cells were significantly increased (P<0.05); the expression levels of Bcl-2 and p-ERK in sh-Derlin-1 group were significantly decreased, while the expression level of Bax was greatly increased (P<0.05). CONCLUSION: The decreased level of Derlin-1 can suppress the proliferation and induce the apoptosis of SW480 cells through suppressing the activity of ERK signaling pathway.

Key words: Derlin-1, human colon cancer cells, proliferation, apoptosis, ERK signaling pathway

CLC Number:

R965.2

ZHAO Li, HUA Xia, TAN Ye. Effects of endoplasmic reticulum associated degradation protein Derlin-1 on the proliferation of human colon cancer cells via suppressing ERK pathway[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(12): 1353-1358.

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