Effects of Astragalus-Angelica combination on VSMC proliferation of vascular intimal hyperplasia in rats

doi:10.12092/j.issn.1009-2501.2018.04.001

Abstract

Abstract:

AIM: To investigate the effects of different Astragalus-Angelica combination on vascular smooth muscle cells (VSMC) phenotype transformation, cell cycle and PI3K/AKT signal pathway of vascular intimal hyperplasia in rats. METHODS: A model of intimal hyperplasia of thoracoabdominal aorta was established by balloon catheter injury in male Sprague-Dawley rat, and the model rats were administrated Astragalus and Angelica with single use or different ratios for 14 days by means of intragastric administration. Then the thoracoabdominal aorta was taken out to analyze the pathological changes of the VSMC, expression of cell cycle and PI3K/Akt signaling pathway related proteins in hyperplastic intima. RESULTS: After balloon injury of aortic endothelium, VSMC phenotype transformation and proliferation can be induced, resulting in intimal hyperplasia. In hyperplastic intima, the expression of SM-actin can be enhanced and the expression of PCNA can be inhibited by the Astragalus and Angelica with single use and Astragalus-Angelica of 1∶1 and 5∶1 combination. The increased expression of cyclinD1 or cyclinE in damaged blood vessels and p-PI3K or p-Akt protein in hyperplastic intima can be inhibited by the Astragalus and Angelica with single use and Astragalus-Angelica of 1∶1 and 5∶1 combination. CONCLUSION: Vascular intimal hyperplasia can be inhibited by Astragalus-Angelica compatilibity. The mechanism may be related to the inhibition of PI3K/Akt signaling pathway activation, which lead to the inhibition of the phenotypic transformation and cell proliferation of VSMC, and then play the role of anti VSMC proliferation.

Key words: Astragalus, Angelica, VSMC, cell cycle, PI3K/Akt

CLC Number:

R965.2

YAN Huifang, XU Hao, PENG Xiwei, ZHU Jiahuan, DENG Changqing. Effects of Astragalus-Angelica combination on VSMC proliferation of vascular intimal hyperplasia in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(4): 361-369.

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