Fig.1 Effect of ionizing radiation on viability of H9C2 cells by using CCK-8 method ($ \overline{x} $±s, n=3) bP<0.05, cP<0.01, compared with 0 Gy group.

Fig.2 The effects of ionizing radiation on myofibrillar skeleton of H9C2 cells were observed by guaninyl penicillamine staining (inverted microscope, 400×)

Fig.3 Effect of ionizing radiation on apoptosis of H9C2 cells with the Hoechst33342 staining (inverted microscope, 200×)

Fig.4 Effect of ionizing radiation on total apoptosis rate of H9C2 cells with the flow cytometry assay ($ \overline{x} $±s, n=3) bP<0.05, cP<0.01, compared with blank group.

Fig.5 Detection of the effect of ionizing radiation on mitochondrial transmembrane potential in H9C2 cells at 24 and 48 hours using JC-1 staining

Fig.6 Effect of ionizing radiation on mitochondrial transmembrane potential of H9C2 cells with the flow cytometry assay ($ \overline{x} $±s, n=3) cP<0.01, compared with blank group; eP<0.05, fP<0.01, compared with 2 Gy group.

Fig.7 Effect of RAS-RH on viability of ionizing radiation-induced H9C2 cells by using CCK-8 method ($ \overline{x} $±s, n=3) cP<0.01, compared with blank group; fP<0.01, compared with model group.

Fig.8 Effect of RAS-RH on muscle silk skeleton of ionizing radiation-induced H9C2 cells by using the ghost closed-loop peptide staining (inverted microscope, 400×)

Fig.9 Effect of RAS-RH on apoptosis of ionizing radiation-induced H9C2 cells by using Hoechst33342 staining (inverted microscope, 400×)

Fig.10 Effect of RAS-RH on ionizing radiation-induced apoptosis in H9C2 cells was determined by flow cytometry ($ \overline{x} $±s, n=3) cP<0.01, compared with blank group; fP<0.01, compared with model group.

Fig.11 Effect of RAS-RH on mitochondrial transmembrane potential of ionizing radiation-induced H9C2 cells by using JC-1 staining (inverted microscope, 400×)

Fig.12 The effect of RAS-RH on the ionizing radiation-induced mitochondrial transmembrane potential in H9C2 cells was examined by JC-1 flow cytometry ($ \overline{x} $±s, n=3) bP<0.05, compared with blank group; eP<0.05, fP<0.01, compared with model group.

Fig.13 Effect of Drp1 and HSP70 proteins expression in ionizing radiation-induced H9C2 cells by using Western blot technology ($ \overline{x} $±s, n=3) bP<0.05, cP<0.01, compared with 0 Gy group.

Fig.14 Effect of RAS-RH on the Drp 1 and HSP70 proteins expression in ionizing radiation-induced H9C2 cells by using Western blot technology ($ \overline{x} $±s, n=3) cP<0.01, compared with blank group; eP<0.05, fP<0.01, compared with model group.