Effect of curcumin on the human colon cancer cells SW480

Abstract

Abstract:

AIM: To study the effect of curcumin on human colon cancer cells SW480. METHODS: Cell viability and the effects of curcumin on the activity of SW480 cells were detected by MTS method. Expressions of β-catenin and TCF4 were detected by Western blot. E-cadherin and Vimentin by real-time qPCR assay in human colon cancer cells SW480. RESULTS:Curcumin could significantly inhibit the proliferation of SW480 cells and the IC50 were (75.75±3.17) μmol/L（12 h）, (21.15±2.47) μmol/L（24 h）, (18.95±1.75) μmol/L（48 h）, (17.93±1.55) μmol/L（72 h）. Curcumin could up-regulate the expression of E-cadherin, and down-regulate the expression of Vimentin, β-catenin and TCF4 in SW480 cells. CONCLUSION: Curcumin can inhibit the the activity of SW480 cells through the Wnt/β-catenin signaling pathway.

Key words: curcumin, SW480 cells, β-catenin, TCF4, inhibition

CLC Number:

R965.2

CHEN Yongcai, ZHOU Bin, CHEN Xuemin, WENG Jinyue. Effect of curcumin on the human colon cancer cells SW480[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(5): 526-530.

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