Abstract: AIM: To explore the genotype differ-ence of CYP3A4 gene from Chinese Han population in East China .METHODS: The CYP3A4 gene cDNA of six samples of adult human liver tissues from Chinese Han population were amplified and identified by RT-PCR and DNA sequencing .RESULTS: One new geno-type of CYP3A4 was identified from six samples.There are three amino acids mutations, i .e .V (GTG) →A (GCG) at 71st amino acid site ;V(GTC) →I(AIC) at 225th amino acid site;W(TGG) →V(GTG) at 393rd amino acid site, and three nucleotide acids lack, i .e . CAG from 671st to 673rd nucleotide acid site. CONCLUSION: one new genotype of CYP3A4 gene is dis-covered for the first time from Chinese Han population in East China.

Key words: P450, CYP3A4, mutation, genotype