Abstract: AIM: To research the inhibitory effect of antihypercoagulability state enzyme(AHCSE) of agkistrodon venom on the hepatocarcinoma in Balb cmice bearing hepatocarcinoma (H22) strain in situ and its possible mechanisms. METHODS: Hepatic cancer H22 model in mice was established on axillary fossa (Balb c) subcutaneouly, to obtain post-passage carcino-tissue and raise on mice livers.All animals were divided into 4 groups, 10 mice each group:control group, low-AHCSE group(0.5 mg/kg), middle-AHCSE group(1.0 mg/kg) and high-AHCSE group(2.0 mg/kg).Different dosages of AHCSE were injected via vena caudalis every three days for three times.To explore therapic efficacy of different dosages of AHCSE on the basis of percent inhibition of neoplastic weight and positive cells of P53 and C-MYC. RESULTS: The experiment of aniso-dosage of AHCSE indicates, percent inhibition of tumor(PIT) was 35.57% in low-AHCSE group (0.5 mg/kg), the PIT in the middle-dosage group(1.0 mg/kg) was 50.38% and in high-AHCSE group (2.0 mg/kg) PIT was 53.89%.The expression of P53 protein was 64, 51 and 45 and the expression of CMYC protein was 46, 38 and 34 in the three dosages groups.Inhibitory effect on tumor was significantly inmiddle-AHCSE and high-AHCSE. CONCLUSION: AHCSE has the significantly inhibitory effect on tumor in dose dependent.AHCSE may inhibit the tumor via down regulation the expression of P53, C-MYC protein.

Key words: agkistrodon venom, mice hepatic cancer(H22), mechanism