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Chinese Journal of Clinical Pharmacology and Therapeutics ›› 2009, Vol. 14 ›› Issue (10): 1128-1132.

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Inducible nitric oxide synthase expression in lung tissue and neutrophil of asthmatic rats and the effects of dexamethasone on them

CHEN Cun-guo1, JIN Hai-hua1, LI Shao-bo1, JIN Xiao-hong1, CHEN Bao-guo1, TONG Xia-sheng2, YE Hui3   

  1. 1Taizhou Hospital Affiliated to Wenzhou Medical College, Linhai 317000, Zhejiang, China;
    2Taizhou Integrated Traditional and Western Medicine Hospital, Wenling 317523, Zhejiang, China;
    3Taizhou First People' s Hospital, Taizhou 318020, Zhejiang, China
  • Received:2009-06-30 Revised:2009-09-16 Published:2020-10-29

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Abstract: AIM: To observe the expression of inducible nitric oxide synthase (iNOS)in neutrophils and lung tissues and explore the roles of peripheral blood neutrophils in the pathogenesis of asthmatic inflammation in asthmatic rats. METHODS: Experimental rats were randomly divided into asthma, control and dexamethasone treated groups.Peripheral blood neutrophils were isolated and the expression of iNOS was detected by immunohistochemistry.The nitric oxide (NO)concentration of bronchoalveolar lavage fluids (BALF)was also determined. RESULTS: iNOS protein of PMN in asthmatic rats (0.122 ±0.017 Optical density)was significantly higher than that in control group (0.076 ±0.014, P <0.01).iNOS protein of PMN in dexamethasone treated group (0.089 ±0.013)was markedly lower than that in asthma group (P <0.01), but there was no statistically significant for the PMN iNOS expression between the dexamethasone treated group and control group.Furthermore, iNOS of bronchial wall in asthma group (0.243 ±0.039)was dramatically higher than that in control group (0.119 ±0.016, P <0.01).iNOS in dexamethasone treated group (0.164 ±0.016)was significantly lower than that in asthma group, but was higher than that in control group (P <0.01, respectively).NO concentration of BALF in asthma group (8.59 ±1.07)ng/mL was significantly higher than control group (3.69 ±1.00 ng/mL, P <0.01).NO concentration of BALF in dexamethasone treated group (4.28 ±0.89)ng/mL was significantly lower than that in asthma group, while no significance was observed between the indexamethasone treated and control group.Moreover, iNOS protein expression of PMN was highly correlated with NO concentration of BALF (n =29, r =0.770, P <0.01), and a similar strong association was established between the bronchial wall iNOS and NO concentration of BALF (n =29, r =0.802,P <0.01). CONCLUSION: iNOS of PMN was elevated in asthmatic rats, which could be partly inhibited by dexamethasone.Our study indicated that an increased iNOS expression of PMN may involved in the pathogenesis of asthma in rats.

Key words: asthma, inducible nitric oxide synthase, nitric oxide, peripheral blood monocytes, rat

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