Experimental study of the relationship between Smad2/3 and Smad7 proteins expressions in blood polymorphonuclear leucocyte and the asthma exacerbation

Abstract

Abstract: AIM: To observe the expressions of Smad2/3 and Smad7 proteins at blood polymorphonuclear leukocyte (PMN) in rats asthma.And to determine whether PMN participate in airway inflammation of asthma via Smads ysterm.METHODS: Rat asthma model was induced by ovalbumin, twenty male Sprague-Danley rats were randomly divided into two groups on average, including asthma group and control group.Blood PMNs were isolated and purified.The expressions of Smad2 3 and Smad7 proteins were detected by immunocytochemical method at blood PMN.RESULTS: The expressions of Smad2 3 protein were significantly higher in asthma group (0.142±0.021, optical density ) than those in control group(0.081±0.011, optical density, P<0.01).But the expressions of Smad7 protein were significantly lower in asthma group(0.125±0.024, optical density) than those in control group(0.257±0.047, optical density, P<0.01).There were significant statistical negtive correlation between the expression of Smad2 3 and Smad7 (n=19, r= -0.891, P<0.01 ).CONCLUSION: Smads system is in imbalanced state in asthma exacerbation.Receptor-regulated Smads is predomination.The synthesis function of PMN about Smad2/3 and Smad7 protein are increased in asthma exacerbation.PMN participated in airway inflammation of asthma may be via Smads systerm.

Key words: asthma, Smads, polymorphonuclear leukocyte, rat

CLC Number:

R965.2

TONG Xia-sheng, LUO Dong-jiao, FANG Hui-ying, FANG Li, WANG En-zhi, CHEN Hao, YE Hui. Experimental study of the relationship between Smad2/3 and Smad7 proteins expressions in blood polymorphonuclear leucocyte and the asthma exacerbation[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(4): 405-409.

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