Abstract: AIM: To investigate the effects of Rosa roxburghii Tratt extract-CL1 on proliferation of gastric carcinoma SGC-7901 cells, and on proliferation and differentiation of human umbilical cord (UCB) CD34+cells.METHODS: The OD value of living cells was measured by MTT test.UCB CD34+cells were isolated by magnetic-activated cell sorting, and surface marker of cells was determined using f low cytometry.Cells were counted by trypan blue dye exclusion test.RESULTS: The inhibitory percentages of CL1 (0.01-10 μmol·L^-1) on growth of SGC-7901 cells were concentration-dependent and time-dependent from 12 to 96 h.After culturing for 14 d,compared with control group, there was a downtrend of the numbers of CL1 10 and 1 μmol·L^-1 but there was no significance(P>0.05) , and there were no difference in the cell number percentage of both expressing CD15 and expressing CD11b.But compared with d 0, the cell number percentage of expressing CD15 were remarkably increased;the cell number percentage of expressing CD11b had an increasing tendency, but it was not significant (P>0.05).CONCLUSION: CL1 has inhibitory effect on growth of SGC-7901 cells in vitro, but does not affect proliferation of CD34+and differentiation of CD34+haematopoietic stem progenitor cells to granulocyte and monocytes.This demonstrates that CL1 has anticancer effect,but does not affect the proliferation and differentiation of haematopoietic stem progenitor cell to granulocytes and monocytes.It may not cause obvious haematopoietic depression.

Key words: Rosa roxburghii Tratt, CL1, gastric carcinoma, heamatopoietic stem progenitor cell, proliferation, differentiation