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Chinese Journal of Clinical Pharmacology and Therapeutics ›› 2006, Vol. 11 ›› Issue (8): 878-882.

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Tumor associated and immune associated gene differential expression by cDNA microarray in rat liver treated with berberine chloride

YU Ai-rong, XIN Hua-wen, WU Xiao-chun, LI Qing, ZHU Min, ZHANG Qin   

  1. Department of Clinical Pharmacology , Wuhan General Hospital , Wuhan 430070 , Hubei , China
  • Received:2006-04-12 Revised:2006-07-11 Online:2006-08-26 Published:2020-11-05

Abstract: AIM: To study the effects of berberine chloride(Ber) on tumor associated and immune associated gene expression profile in rat liver.METHODS: After orally treated with berberine chloride for 6 days, the rats were sacrificed and the livers were taken out to extract total RNA.mRNA from negative control grouPand Ber grouPwere labeled with Cy3-dCTPand Cy5-dCTPrespectively.These probes were hybridized with cDNA microarray.The acquired image was scanned and analyzed by GenePix Pro 3.0 software.The intensity ratio of Cy5 to Cy3 about each spot was calculated.RESULTS: Among 4096 genes, 236 genes exhibited differential expressions in grouPtreated with Ber.22 tumor associated and immune associated genes were differentially expressed with 9 genes up-regulated and 13 genes down-regulated.The genes up-regulated included CYP17, kangai 1, opioidbinding protein/cell adhesion molecule-like (Opcml) , interleukin 6, kidney-specific protein, interleukin 2 receptor and alpha-2-macroglobulin.The genes down-regulated included cytochrome P450 2E1, testicular cell adhesion molecule,S-adenosyl-L-methionine decarboxylase , asialoglycoprotein receptor, tumor protein translationally-controlled 1 (Tpt1) , CTL target antigen and S100 calcium-binding protein A9.CONCLUSION: The anti-cancer effects and immunoregulatory function of Ber may be related with differential expressions of some tumor associated and immune associated genes.

Key words: berberine chloride, tumor, immune, cDNA microarray

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