Effects of crocetin on doxorubicin-induced damage in rats with myocardial mitochondria

Abstract

Abstract: AIM: To study the protective effects of crocetin on the doxorubicin-induced damage in rats with myocardial mitochondria. METHODS: Rats were given intraperitoneal injection of doxorubicin to induce cardiotoxicity. After continuous oral administration of crocetin, the rats were sacrificed, and myocardial mitochondria were isolated. The mitochondrial membrane potential (MMP), $O^{-}_{2}$ .production, the activity of respiratory chain complex IV and glutathione peroxidase (GSH-PX), and DNA fragmentation were determined. The expression level of COII gene was determined through RT-PCR. RESULTS: Crocetin increased the activity of respiratory chain complex IV and GSH-PX, MMP and the expression level of COII and decreased DNA fragmentation and superoxide anion radical $O^{-}_{2}$ .production in rats with myocardial mitochondria. CONCLUSION: Crocetin may decrease the damage in rat myocardial mitochondria induced by doxorubicin.

Key words: crocetin, doxorubicin, mitochondria, cytochrome C oxidase subunit II (COII)

CLC Number:

R965

LI Wen-na, QIAN Zhi-yu. Effects of crocetin on doxorubicin-induced damage in rats with myocardial mitochondria[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(7): 764-767.

References

1 De Beer EL, Bottone AE, Voest EE. Doxorubicin and mechanical performance of cardiac trabeculae after acute and chronic treatment:a review[J]. Euro J Pharmacol, 2001; 415:1-11
2 徐霆, 江文, 李生广, 赵保路, 忻文娟, 林治焕.镁离子对阿霉素线粒体毒性的防护作用[J]. 生物物理学报, 1995; 22: 614-8
3 李文娜, 钱之玉.西红花酸对多柔比星致大鼠心脏毒性的影响[J]. 中国新药杂志, 2005;(印刷中)
4 Solem LE, Heller LJ,Wallace KB.Dose-dependent increase in sensitivity to calcium-induced mitochondrial dysfunction and cardiomyocyte cell injury by doxorubicin[J]. J Mol Cell Cardiol, 1996;28: 1023-32
5 谭勇, 梁清华, 李霞玲, 张花先, 李春燕, 包太成, 等.平肝熄风汤对脑出血大鼠海马线粒体膜电位的影响[J]. 中南大学学报(医学版), 2004; 29:35-8
6 邱小忠, 陈瑗, 周玫, 张稳柱.安定导致小鼠大脑的线粒体氧应激损伤[J]. 第一军医大学学报, 2001;21: 279-81
7 Veitch K, Hombroeckx A, Caucheteux D, Pouleur H, HUE L. Golbal ischaemia induces a biphasic response of the mitochondrial respiratory chain[J]. Biochem J, 1992;281: 709-15
8 冯卫红, 刘耕陶.N-甲基-4-苯基1, 2, 3, 6-四氢吡啶及多巴胺体外引起大鼠脑线粒体损伤作用[J]. 药学学报,2000;35:11-7
9 Papadopoulou LC, Theophilidis G, Thomopoulos GN, Tsiftsoglou AS.Structural and functional impairment of mitochondrial in adriamycin-induced cardiomyopathy in mice: suppression of cytochrome c oxidase II gene expression[J]. Biochem Pharmacol, 1999;57:481-9
10 Ji LL, Mitchell EW.Effects of adriamycin on heart mitochondrial function in rested and exercised rats [J]. Biochem Pharmacol, 1994;47:877-85
11 Praet M, Ruysschaert JM.In vivo and in vitro mitochondrial membrane damage induced in mice by adriamycin and derivatives[J]. Biochim Biophys Acta, 1993; 1149: 79-85
12 Papadopoulou LC, Tsiftsoglou AS.Mitochondrial cytochrome C oxidase as a target site for daunomycin in K-562 cells and heart tissue[J]. Cancer Res, 1993; 53:1072-8
13 栾荣华, 马梃光.阿霉素心脏毒性作用机制的研究进展[J]. 心血管学进展, 1998; 19:108-10
14 李斯达.抗氧化剂拮抗阿霉素心肌毒性的研究近况[J]. 中国航天工业医药, 2001;3: 73-4

[1]	HU Kangyi, CAO Linzhong, WAN Chaochao, SHANG Zhengya, YANG Xiaorui, ZHANG Yongjie. Research progress of traditional Chinese medicine regulating mitochondrial function in the treatment of steroid-induced femoral head necrosis [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(6): 688-696.
[2]	BAI Jia, YANG Hong, LI Lingling, ZHANG Yangyang, YANG Ying, LV Haihong. Study on the effect of vitamin D on diabetic neuropathy through mitochondrial/autophagy pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(2): 214-219.
[3]	ZHANG Li, WANG Jin, LI Huizi, PENG Hui, HE Jun, PENG Shuangqing, GUO Jiabin. Role of Nrf2 pathway in flutamide-induced mitochondrial biogenesis [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(5): 498-504.
[4]	DONG Wei, HUANG Xiaoying, XIE Bingbin, TANG Xilan, LI Hongming, QIU Yumei, ZHAO Guowei, LIANG Xinli. Effects of oxypeucedanin on the resistance of breast cancer MCF-7/DOX cells to doxorubicin [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(3): 260-266.
[5]	QUAN Haiyan, JIANG Xing, HE Lu. Recent progress of mitophagy in hepatic insulin resistance [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(2): 198-204.
[6]	WANG Xiaolin, GAO Pan, ZOU Yunzeng. Association between mitochondrial oxidative stress and diabetic cardiomyopathy [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2021, 26(9): 1080-1085.
[7]	DING Xueming, CHEN Tianpeng, XU Qiang, HE Huan, HE Ming. Tetramethylpyrazine attenuates doxorubicin induced cardiotoxicity through 14-3-3γ/Bcl-2 [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2021, 26(4): 361-367.
[8]	JIN Jing, CHENG Hong. Research progress of mitochondrial energy metabolism involved in the pathogenesis of depression [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2021, 26(10): 1193-1199.
[9]	ZHANG Pinghu, ZHANG Ling, LIU Changxiao. Research progress of mitochondrial toxicity evaluation model for nucleoside drugs [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2020, 25(9): 1059-1065.
[10]	CHEN Yi, CAO Hua, CAI Lingfang, CHEN Ben, CHEN Mingkun. Lidocaine inhibits the malignant proliferation, invasion and regulates the mitochondrial respiration of osteosarcoma MG-63 cells [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2020, 25(5): 519-526.
[11]	MA Ji, CUI Zhao, WANG Huajing, LI Shuo, QIN Tingting, LI Canghai, JIANG Tingliang. Effects of antimalarial drugs on mitochondrial respiration in erythrocytic stages of Plasmodium falciparum 3D7 [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2020, 25(11): 1201-1213.
[12]	DUAN Jinning, XIANG Changqing, QIAN Aihong, LI Jin, CHEN Xiuquan, HAN Yongfeng, HUANG Na, FAN Lili. Saponins from Rhizoma Panacis Majoris protect against cerebral ischemia and reperfusion injury through modulation of PI3K/Akt signaling pathway in mice [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(7): 750-758.
[13]	CAI Miaoguo, SHAO Wei, YU Huijun, HONG Ye, SHI Lili. Effects of regulation ROS on the pain and the protein of Pink1 in spinal of SNI mice and its mechannism [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(11): 1263-1268.
[14]	LIU lei, LI Yixiao, WAN Bo, ZENG Qing. miR-200c induces resistance to doxorubicin through the Wnt/beta-catenin signaling pathway in bladder cancer cells [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(5): 510-516.
[15]	CAI Mingmin，YAO Lan，ZHANG Jingwei，ZHOU Fang，WANG Guangji. Research progress in drug-induced acute kidney injury based on mitochondrial damage [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(5): 578-585.