Effects of crocetin on doxorubicin-induced damage in rats with myocardial mitochondria

Abstract

Abstract: AIM: To study the protective effects of crocetin on the doxorubicin-induced damage in rats with myocardial mitochondria. METHODS: Rats were given intraperitoneal injection of doxorubicin to induce cardiotoxicity. After continuous oral administration of crocetin, the rats were sacrificed, and myocardial mitochondria were isolated. The mitochondrial membrane potential (MMP), $O^{-}_{2}$.production, the activity of respiratory chain complex IV and glutathione peroxidase (GSH-PX), and DNA fragmentation were determined. The expression level of COII gene was determined through RT-PCR. RESULTS: Crocetin increased the activity of respiratory chain complex IV and GSH-PX, MMP and the expression level of COII and decreased DNA fragmentation and superoxide anion radical $O^{-}_{2}$.production in rats with myocardial mitochondria. CONCLUSION: Crocetin may decrease the damage in rat myocardial mitochondria induced by doxorubicin.

Key words: crocetin, doxorubicin, mitochondria, cytochrome C oxidase subunit II (COII)

CLC Number:

R965

LI Wen-na, QIAN Zhi-yu. Effects of crocetin on doxorubicin-induced damage in rats with myocardial mitochondria[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(7): 764-767.

References

1 De Beer EL, Bottone AE, Voest EE. Doxorubicin and mechanical performance of cardiac trabeculae after acute and chronic treatment:a review[J]. Euro J Pharmacol, 2001; 415:1-11
2 徐霆, 江文, 李生广, 赵保路, 忻文娟, 林治焕.镁离子对阿霉素线粒体毒性的防护作用[J]. 生物物理学报, 1995; 22: 614-8
3 李文娜, 钱之玉.西红花酸对多柔比星致大鼠心脏毒性的影响[J]. 中国新药杂志, 2005;(印刷中)
4 Solem LE, Heller LJ,Wallace KB.Dose-dependent increase in sensitivity to calcium-induced mitochondrial dysfunction and cardiomyocyte cell injury by doxorubicin[J]. J Mol Cell Cardiol, 1996;28: 1023-32
5 谭勇, 梁清华, 李霞玲, 张花先, 李春燕, 包太成, 等.平肝熄风汤对脑出血大鼠海马线粒体膜电位的影响[J]. 中南大学学报(医学版), 2004; 29:35-8
6 邱小忠, 陈瑗, 周玫, 张稳柱.安定导致小鼠大脑的线粒体氧应激损伤[J]. 第一军医大学学报, 2001;21: 279-81
7 Veitch K, Hombroeckx A, Caucheteux D, Pouleur H, HUE L. Golbal ischaemia induces a biphasic response of the mitochondrial respiratory chain[J]. Biochem J, 1992;281: 709-15
8 冯卫红, 刘耕陶.N-甲基-4-苯基1, 2, 3, 6-四氢吡啶及多巴胺体外引起大鼠脑线粒体损伤作用[J]. 药学学报,2000;35:11-7
9 Papadopoulou LC, Theophilidis G, Thomopoulos GN, Tsiftsoglou AS.Structural and functional impairment of mitochondrial in adriamycin-induced cardiomyopathy in mice: suppression of cytochrome c oxidase II gene expression[J]. Biochem Pharmacol, 1999;57:481-9
10 Ji LL, Mitchell EW.Effects of adriamycin on heart mitochondrial function in rested and exercised rats [J]. Biochem Pharmacol, 1994;47:877-85
11 Praet M, Ruysschaert JM.In vivo and in vitro mitochondrial membrane damage induced in mice by adriamycin and derivatives[J]. Biochim Biophys Acta, 1993; 1149: 79-85
12 Papadopoulou LC, Tsiftsoglou AS.Mitochondrial cytochrome C oxidase as a target site for daunomycin in K-562 cells and heart tissue[J]. Cancer Res, 1993; 53:1072-8
13 栾荣华, 马梃光.阿霉素心脏毒性作用机制的研究进展[J]. 心血管学进展, 1998; 19:108-10
14 李斯达.抗氧化剂拮抗阿霉素心肌毒性的研究近况[J]. 中国航天工业医药, 2001;3: 73-4

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