Abstract: AIM: To improve the acute cumulative death rates method (ACD method) in animal by oral administration.METHODS: A mathematic model was established to evaluate the dose-toxic effect relationship by twice oral administration and the experimental steps were improved too.The methodological quality was explored using the simulation data from computer program and the real experimental date from the reference paper.RESULTS: The results showed that the experimental data could be fitted to its theoretical data from LD₅/2 to LD₉₅/2.Concentration-time curve after po ordinary powder of Semen Strychni in mice were fitted to a one-compartment with T_1/2(ka)=1.136 h, T_1/2(ke)=7.100 h, and T_max= 3.576 h.CONCLUSION: The improved ACD method can be used in the pharmacokinetics of TCM by oral administration.

Key words: traditional Chinese medicine, pharmacokinetics, acute cumulative death rates, computer simulator, absorption