Inhibitory effect of ginsenoside Rg1 on BGC-823 human gastric cancer cell line

Abstract

Abstract: AIM: To observe the influences of Ginsenoside Rg1 on the activity, proliferation, the expression of Bax-2 and caspase-3 and morphology of BGC-823 gastric cancer cell line, and investigate its pharmacological mechanisms. METHODS: Cells which were stayed in logarithmic growth were used for the experiments, the cells were interfered with different concentration of Ginsenoside Rg1, the methods of growth curve, M'IT assay, protein content assay Real-time PCR and morphological observation were used to investigate the effects of Ginsenoside Rg1 on BGC-823 cells. RESULTS: Ginsenoside Rg1 had potent inhibitory effect on BGC-823 cell line in a dose-dependent and time-dependent manner. Ginsenoside Rg1 had significant cytotoxic effect to BGC-823 cells, and its half maximal inhibitory concentration (IC₅₀) is 29.56 mg/L. Ginsenoside Rg1 decreased the amount of protein in BGC-823 cells, improved the expression of Bax-2、caspase-3, and caused the shrinkage of cells, the cytoplasm decreased, and the color was salmon pink; the chromatin condensed and the color was intense violet; the cellular nucleuses were condensed or broken into round granules. CONCLUSION: Ginsenoside Rg1 obviously inhibited the cell proliferation, decreased its activity, showed conspicuous anti-tumor effect on BGC-823 cells. The anti-tumor effect may be related to the inhibition effect to tumor cell protein synthesis, and promote the apoptosis of BGC-823 cells.

Key words: Ginsenoside Rg1, Gastric cancer, BGC-823, Cell proliferation, Apoptosis

CLC Number:

R966

ZHAO Bao-sheng, LIU Yang, XU Tun-hai. Inhibitory effect of ginsenoside Rg1 on BGC-823 human gastric cancer cell line[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2011, 16(4): 361-365.

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