Relaxation effects of dauricine on isolated tracheal smooth muscles of guinea pigs

Abstract

Abstract: AIM: To investigate the relaxation effects of Dauricine (Dau) on isolated tracheal smooth muscles of guinea pigs. METHODS: We made tracheal pieces of guinea pigs, and used experimental methods in isolated organs, recorded the relaxation effect of Dau for isolated tracheal smooth muscles of guinea pigs, and also observed the relaxation effect's changes of Dau for isolated tracheal smooth muscles of guinea pigs in different channel and receptor blockers. RESULTS: Dau had a significant relaxing effect on isolated tracheal smooth muscles of guinea pigs, and the relaxation effect had a significant concentration-dependent between 60 to 120 μmol/L concentrations (P<0.05). The relaxation of isolated guinea pig tracheal smooth muscle of nifedipine plus Dau group was weaker than that of Dau group between 80 to 160 μmol/L concentrations (P<0.05). The relaxation of isolated guinea pig tracheal smooth muscle of propranolol plus Dau group was weaker than that of Dau group between 60 to 120 μmol/L concentrations (P<0.05), but the relaxation of isolated guinea pig tracheal smooth muscle of propranolol plus Dau group was similar compared to Dau group between 120 to 160 μmol/L concentrations(P>0.05). CONCLUSION: Dau has a significant relaxing effect on isolated tracheal smooth muscles of guinea pigs and it may be related with β₂ receptors and inhibiting calcium channel.

Key words: Dauricine, Isolated tracheal smooth muscles of guinea pigs, Calcium channel, β₂ receptor

CLC Number:

R285.5

SHEN Bai-ru, ZENG Zhi-lin, XU Ji-de, LIU-Si, XU Xiao-yang, ZHU Wei-si, LI Yin, JIAN Wen-hua. Relaxation effects of dauricine on isolated tracheal smooth muscles of guinea pigs[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2011, 16(10): 1110-1113.

References

[1] 王德娟, 王栋. 蝙蝠葛酚性碱药理作用研究进展[J]. 中医药学报, 2008, 36(5): 44-45.
[2] Xiao YL, Qian L, Dong MW, et al. Validated liquid chromatography-tandem mass spectrometry method for quantitative determination of dauricine in human plasma and its application to pharmacokinetic study[J]. J Chromatogr B Analyt Technol Biomed Life Sci, 2010, 878(15/16): 1199- 1203.
[3] Yang XY, Liu QN, Zhang L, et al. Neuroprotective effect of dauricine after transient middle cerebral artery occlusion in rats: involvement of Bcl-2 family proteins[J]. Am J Chin Med, 2010, 38(2): 307- 318.
[4] 邓文龙. 山豆根[M] //王浴生, 邓文龙, 薛春生. 中药药理与应用. 2版. 北京: 人民卫生出版社, 93-101.
[5] 杨燕秋, 黎锐发, 许继德, 等. 川芎嗪注射液对离体豚鼠气管平滑肌作用机制探讨[J]. 中国临床药理学与治疗学, 2004, 9(5): 558-560.
[6] 张彦周, 冯义柏, 黄恺, 等. 蝙蝠葛碱对心肌去甲肾上腺素出胞释放的影响[J]. 中国药理学通报, 1998, 14(1): 45-47.
[7] Xia JS,Tu H,Li Z, et al. Dauricine suppressed CsCI-induced early after depolarizations and triggered arrhythmiasin rabbit heart in vivo[J]. Acta Pharmacologica Sinica, 1999, 20(6): 513-516.
[8] Liu QN, Zhang L, Gong PL, et al. Inhibitory effects of dauricine on early afterdepolarizations and L-type calcium current[J]. Can J Physiol Pharmacol, 2009, 87(11): 954-962.
[9] Qian JQ. Cardiovascular pharmacological effects of bisbenzylisoquinoline alkaloid derivatives[J]. Acta Pharmacol Sin, 2002, 23(12): 1086-1092.
[10] 朱接全, 吴绍光, 胡崇家. 蝙蝠葛碱对四种血管平滑肌的作用[J]. 同济医科大学学报, 1987, (5): 361.
[11] 何玲, 刘国卿, 王金晞, 等. 蝙蝠葛苏林碱对PC1₂细胞内游离钙浓度的影响[J]. 中国药理学报, 1997, 13(5): 416-419.