Influence of rhein intervention on the expression of HGF and BMP7 in renal tissue of rats with chronical allograft nephropathy

Abstract

Abstract: AIM: To investigate the expression of HGF and BMP7 in renal tissue of rats with chronical allograft nephropathy (CAN) and the influence of rhein intervention on their expression. METHODS: We used Fisher rats as donors and Lewis rats as recipients to establish the model of chronic allograft nephropathy. 30 rats with transplanting kidneys were randomly divided into two groups: 16 rats in blank group and 14 rats in rhein intervention group. 5 normal lewis rats were as controls. The intervention group was given rhein oral solution 100 mg·kg^-1·d^-1 by gavage after transplantation. The blank group and control group were given 0.5% solution of sodium carboxymethyl cellulose. The blood and urine sample were collected at 4 weeks, 8 weeks and 16 weeks after transplantation respectively and renal function and total urine protein were examined. Half of rats in each group were killed at 8 weeks and 16 weeks for examining the renal pathology. And then we used immunohistochemical methods and real-time quantitative PCR to detect the protein expression levels and mRNA expression levels of HGF, BMP7 and transforming growth factor β₁(TGF-β₁) in CAN rats kidney tissues. RESULTS: (1) The rhein intervention group was significantly different from blank group(P<0.05 ). Rhein can improve renal functions and significantly reduce the grade of renal fibrosis and interstitial inflammation(P<0.05 ); (2) The levels of BMP7 and HGF were markedly elevated in renal tissues of rhein intervention group(P<0.05 ). However, the expression of TGF-β₁ was similar between these two groups. CONCLUSION: Rhein can improve renal functions and reduce the grade of renal fibrosis and interstitial inflammation, which may associate with that rhein plays a role in anti-inflammation, inducing the expression of BMP7 and HGF.

Key words: Rhein, Chronical allograft nephropathy, Renal fibrosis, Mechanism

CLC Number:

R965.2

SU Jian, YIN Li-ping, ZHANG Xin, LI Bi-bo, LIU Li, LI Hui. Influence of rhein intervention on the expression of HGF and BMP7 in renal tissue of rats with chronical allograft nephropathy[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2011, 16(10): 1114-1120.

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