Repair effect of human tissue kallikrein on sciatic nerve injury of rats

Abstract

Abstract: AIM: To study the repair effect of human tissue kallikrein (HTK) on sciatic nervous of rats.METHODS: Thirty-six adult male SD rats weighted 180-220 g were randomly divided into 3 groups. The control group was just injected with 2 mL salt water by caudal vain every day. The SM group was injected with methylprednisolone 30 mg/kg(2 mL)by caudal vain every day. The HTK group was injected with human tissue kallikrein 17.5×10^-3 PNAU/kg (2 mL) by caudal vain every day. The sciatic functional index (SFI) at the day 1,3,5,7,9,11 and 13 after operation in all rats were calculated. The nerve conduction velocity of the sciatic nerve at the 14^th day postoperation was observed.RESULTS: All rats showed -100 at day 1 and 3. The HTK group and the SM group showed a statistically significant improvement over the control group from the 3^th day postoperation. The NCV of the HTK group and the SM group were greater than the control group (P<0.05).CONCLUSION: The repair effect of human tissue kallikrein (HTK) on sciatic nervous of rats was effective.

Key words: Human tissue kallikrein, Sciatic nervous injury, Hormone, Repairation

CLC Number:

R965.2

GENG Wu-jun , LIU Jun , TANG Hong-li, MO Yun-chang , WANG Dan-dan, WANG Jun-lu. Repair effect of human tissue kallikrein on sciatic nerve injury of rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(6): 610-613.

References

[1] 徐建广,顾玉东.大鼠坐骨神经显微解剖及其意义[J].上海医学,1999,22(3):154-156.
[2] Luis AL, Amado S, Geuna S, et al. Long-term functional and morphological assessment of a standardized rat sciatic nerve crush injury with a non-serrated clamp[J]. Neurosci Methods,2007, 163(1): 92-104.
[3] Sun W, Sun C, Lin H, et al. The effect of collagen-binding NGF-beta on the promotion of sciatic nerve regeneration in a rat sciatic nerve crush injury model[J]. Biomaterials, 2009, 30(27): 4649-4656.
[4] Aloe L. Rita Levi-Montalcini: the discovery of nerve growth factor and modern neurobiology[J]. Trends Cell Biol,2004, 14(7): 395-399.
[5] Wagner S, Kalb P, Lukosava M, et al. Activation of the tissue kallikrein-kinin system in stroke[J]. J Neurol Sci,2002, 202(12): 75-76.
[6] 田德虎,米立新,赵峰.周围神经损伤的物理治疗[J].中国康复医学杂志,2004,19(3):239.
[7] Karchewski LA, Gratto KA, Wetmore C, et al. Dynamic patterns of BDNF expression in injured sensory neurons: differential modulation by NGF and NT-3[J]. Eur J Neurosci,2002, 16(8): 1449-1462.
[8] 张奎渤,刘辉,郑召民.甲基强的松龙在脊髓损伤中应用的回顾与现状[J].脊柱外科杂志,2008,6(3):181-183.
[9] 葛志红,张丽萍,张静慧,等.甲强龙联合复方樟柳碱治疗视神经炎的疗效观察[J].中国实用医药,2008,36:33-34.
[10]Bracken MB,Shcpard MJ,Holford TR,et al.Administration of methyIprednisolone for 24 or 48 hours or tirilazad mesylate for 48 hours in the treatment of acute spinal cord injury,Results of the Third National Acute Spinal Cord Injury Randomized Controlled Trial[J].JAMA,1997,277(20):1597-1604.
[11]Fukaya C,Katayama Y,Kasai M,et al.Evaluation of time-dependent spread of tissue damage in experimental spinal cord injury by killed end evoked potendial:effect of high dose methylprednisolone[J].J Neurosurg,2003,98(Supp 1):56-62.
[12]刘珺,王均炉,胡祥鹏,等.人组织激肽释放酶对周围神经系统损伤后的修复效应[J].中国临床药理学与治疗学,2009,14(12):55-57.
[13]王均炉,周乐平,装圣林.人组织激肽释放酶对兔脑血管痉挛的影响[J].中华医学杂志,2008,88(29):2069-2072.

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