Therapeutic effects of 1-Deoxynojirimycin in mulberry leaves on rats with diabetic nephropathy

doi:10.12092/j.issn.1009-2501.2018.05.006

Abstract

Abstract:

AIM: To explore the effect of 1-deoxynojirimycin (DNJ) from mulberry leaves on blood glucose and kidney injury on rats with diabetic nephropathy by investigating the effect DNJ on delay the pathogenesis of diabetic nephropathy. METHODS: Diabetic nephropathy model were established by intraperitoneal injection of streptozotocin (STZ, 35 mg/kg) in male SD rats, then the animals were randomly divided into 3 groups. Rats in low and high dose DNJ groups were given 10, 25 mg/kg DNJ for 6 weeks, and no drug was given to rats in model group. SD male rats were included in experiment as normal control group. Fasting blood glucose (FBG) were detected at the 0, 4th, 6th, 12th week, and an oral glucose tolerance (OGTT) was performed at the end of 6th week after drug intervention. The body mass, urine albumin/creatinine ratio, kidney mass, serum urea nitrogen (BUN) and serum creatinine (Scr) were detected. Histopathological changes of kidney were observed by hematoxylin-eosin (HE) staining. RESULTS: Compared with model group, intragastric administration of low dose (10 mg/kg) or high dose (25 mg/kg) of DNJ reduced blood glucose at 4th and 6th week in diabetic rats, glucose tolerance was also significantly improved at 6th week. After intragastric administration of low dose (10 mg/kg) or high dose (25 mg /kg) of DNJ once daily for 6 weeks, both the renal hypertrophy index and urine albumin/creatinine ratio of rats with diabetic nephropathy were lower than that of model group. Low dose of DNJ reduced the level of Scr,while high dose of DNJ could decrease both the levels of Scr and BUN in diabetic nephropathy rats. By observing the pathological sections of renal tissue, the renal injury of rats with diabetic nephropathy were significantly attenuated with low or high dose of DNJ treatment. CONCLUSION: The DNJ extracted from mulberry leaves reduce blood glucose and attenuate renal damage in rats with diabetic nephropathy, and may delay the pathogenesis of diabetic nephropathy.

Key words: diabetic nephropathy, 1-Deoxynojirimycin (DNJ), rat, blood glucose (BG)

CLC Number:

R965.2

YAO Jia, QIAO Di, GUO Xin, CUI Changmeng. Therapeutic effects of 1-Deoxynojirimycin in mulberry leaves on rats with diabetic nephropathy[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(5): 517-523.

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