Study on analgesic effect of active component from crude cobra venom in Wannan Area

Abstract

Abstract: AIM: To study on analgesic effect of active component from crude cobra venom in Wannan Area(CVAF) and investigate its possible mechanism of analgesic effect.METHODS: Inflammatory pain model of rat (CFA group) was established using CFA (150 μL, s.c.). 40 male SD rats were randomly divided into 4 groups (n=10): NC, CFA, CFA+CVAF and CFA+Morphine groups. Then another 36 male SD rats were randomly divided into 6 groups (n=6): NC, CFA, CFA+CVAF, CFA+Atr+CVAF, CFA+Nal+CVAF and CFA+L-NAME+CVAF groups, the changes of thermal withdrawal latency (TWL) and mechanical withdrawal threshold (MWT), induced by CVAF, were determined by Spurs pain and mechanical pressure methods, respectively. The levels of interleukin (IL)-1 and tumor necrosis factor (TNF)-α in spinal cord were detected by enzyme-linked immunosorbent assay (ELISA). Analgesic activity of CVAF was also evaluated in the CFA group after administrating with atropine, naloxone or L-NAME.RESULTS: Compared with NC group, TWL and MWT were decreased from 1st day to 14th day in CFA group; the levels of IL-1 and TNF-α from spinal cord in CFA group were significantly increased (P<0.01). Compared with the CFA group,the TWL and MWT were increased after injecting CVAF,thermal hyperalgesia and mechanical allodynia were also improved simultaneously,the levels of IL-1 and TNF-α were decreased (P<0.01), the result showed CVAF played a role in analgesic activity via inhibition of inflammatory factor.Compared with CFA+CVAF group, atropine or naloxone could return the increased TWL and MWT by CVAF (P<0.01). The results indicated that both opioid peptide receptor system and cholinergic receptor system could be related to analgesic effect of CVAF for inflammatory pain.CONCLUSION: CVAF plays a role in analgesic effect with inflammatory pain through inhibition of inflammatory factor. Both opioid peptide receptor system and cholinergic receptor system are partially involved in the analgesic effect of CVAF for inflammatory pain.

Key words: Cobra venom, Separation purification, Rat, Analgesic mechanism

CLC Number:

R965.2

WANG Hai-hua, ZHANG Gen-bao, MIN Zhi-xue, HUANG Lu, CUI Feng-juan. Study on analgesic effect of active component from crude cobra venom in Wannan Area[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(10): 1093-1099.

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