Protective effect of total Flavonoid C-glycosides from Abrus mollis extract on fatty liver induced by DL-Ethionine in mice

Abstract

Abstract: AIM: To study the effect of total flavonoid C-glycosides from Abrus mollis extract (AME) on lipid dysregulation induced by Ethionine (DL-E).METHODS: ICR mice were randomly assigned to four groups (n=8): Control, DL-E, AME and AME+DL-E groups. Mice were gavaged with AME (200 mg/kg) for 7 days. At day 7, mice of DL-E and AME+DL-E group were gavaged with DL-E (250 mg/kg), other two groups were treated with vehicle control (0.2% CMC-Na). Serum and liver tissues were collected 22 h after administration of DL-E. The contents of hepatic lipids, serum lipids and ALT, AST levels were measured. The expressions of key genes involved in lipid metabolism were also observed by real-time PCR.RESULTS: The results indicated that AME provided significant protection against fatty liver by inhibiting the elevation of serum lipids, serum transaminase, reducing hepatic TG and TC accumulation, ameliorating the severity of pathological changes. Furthermore, AME significantly decreased DL-E-induced SREBP-1, FAS and ACC1 high expressions, which resulting in the lipid homeostasis. The down-regulation of CPT-1α and PPARα that reflect fatty acid metabolism induced by DL-E were reversed by AME treatment.CONCLUSION: The results indicated AME has hepatoprotective effect on Ethionine-induced fatty liver in mice and the effect was mostly due to the regulation on lipid synthesis and fatty acid metabolism.

Key words: Abrus mollis, Liver injury, Lipid metabolism, SREBP, FAS, PPARα

CLC Number:

R965.2

WANG Yun, CHEN Mi, JIANG Zhen-zhou, WANG Hao, ZHANG Lu-yong. Protective effect of total Flavonoid C-glycosides from Abrus mollis extract on fatty liver induced by DL-Ethionine in mice[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(1): 1-7.

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