Research on relationship between the progression of chronic obstructive pulmonary disease and the change of MRP1 function in lung bronchial epithelium

Abstract

Abstract: AIM: To study the relationship between different progressions of chronic obstructive pulmonary disease (COPD) and the change of MRP1 function in lung bronchial epithelium.METHODS: Making stimulation with tobacco and papain to establish COPD rat model. According to the principle of random, the rats were divided into the normal group, different course of COPD groups, probenecid control group and inhibitor groups.The inhibitor groups were given the MRP1 inhibitor probenecid with the establishing COPD rat model. The function of lung all the rats were evaluated. The permeability and integrity of the lung epithelial cells of all the rats were measured, except the inhibitor group. The phenolsulfonphthalein concentration in bronchoalveolar lavage fluid (BALF) and plasma was determined and the concentration of in BALF/plasma ratio was calculated to evaluate the COPD lung bronchial epithelial MRP1 function.RESULTS: With the development of disease, the lung functions of the rats in different progression of COPD were gradually reduced.There was a significant difference in 45 days later. With the process of COPD, the ratio of phenolsulfonphthalein concentration in BALF and plasma were gradually reduced, but the lung epithelial cell permeability in each groups had not changed and the integrity of the lung epithelial cells was fine.Compared with the normal groups, lung function of the inhibitor group (30 days) was significantly reduced. There was a significant difference in FEV0.3%/FVC between the different courses of COPD groups and inhibitor groups in 60 days.CONCLUSION: There is a certain correlation between the process of COPD and changes of MRP1 function in lung bronchial epithelial. With the progression of COPD, the function of MRP1 is gradually reduced in lung bronchial epithelium. Inhibiting the function of MRP1 can aggravate the lung function of COPD.

Key words: COPD, Progression, MRP1, Function, Correlation

CLC Number:

R965.2

WANG Chen-yin, TAO Xiu-hua, ZHANG Xuan,WANG Dian-lei, CHEN Jin-pei, YANG Li-li, CAO Yin, WANG Shan-shan. Research on relationship between the progression of chronic obstructive pulmonary disease and the change of MRP1 function in lung bronchial epithelium[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(1): 8-14.

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