Effects of norepinephrine on proliferation, migration and invasion of human lung carcinoma cell line H460

Abstract

Abstract: AIM: To investigate the effect of norepinephrine (NE) on proliferation, migration and invasion of human lung cancer cell line H460. METHODS: Human lung cancer cell line H460 were cultured in vitro and treated with NE, then CCK-8 mehod, scratch wound healing assay and transwell assay were applied to measure cell proliferation, migration and invasion. The expression of p38MAPK phosphorylation (Phos-p38) were examined by Western blotting. RESULTS: Compared with the control, 10 μmol/L NE significantly enhanced proliferation, migration and invasion of human lung cancer cell line H460, and elevated p38MAPK phosphorylation level (P<0.01). These effects were completely reversed by β-adrenergic receptor (β-AR) antagonist propranolol (PRO) or p38MAPK inhibitor U0126. CONCLUSION: NE could stimulate lung cancer cell proliferation, migration and invasion, its mechanism may be related with activation of P38MAPK pathway.

Key words: norepinephrine, lung cancer, proliferation, migration, invasion

CLC Number:

R965.2

LONG Xiao-li, ZHANG Yao-heng, LU Wei-qin, LING Ze-yi. Effects of norepinephrine on proliferation, migration and invasion of human lung carcinoma cell line H460[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(11): 1217-1221.

References 14

[1]	Mancino M, Ametller E, Gascón P, et al.The neuronal influence on tumor progression[J]. Biochim Biophys Acta, 2011, 1816(2) :105-118.
[2]	丛吕盛,于甬华.心理神经免疫学在肿瘤学研究中的进展[J]. 中华肿瘤防治杂志, 2008, 15(8) :635-638.
[3]	Antoni MH, Lutgendorf SK, Cole SW, et al.The influence of bio-behavioural factors on tumor biocology:pathways and mechanisms[J]. Nat Rev Cancer, 2006, 6(3) : 240-248.
[4]	Barron TI, Connolly RM, Sharp L, et al.Beta blockers and breast cancer mortality: a population- based study[J]. J Clin Oncol, 2011, 29(19) :2635-2644.
[5]	Gungor C, Zander H, Gebauer F.Replicative stress-induced midkine expression and secretion activates notch signaling and is linked to epithelial-mesenchymal transition and cchemoresistance in pancreatic cancer[J]. Gastroenterology, 2011, 140(5) :S1044-1044.
[6]	Yang EV,Sood AK,Chen M,et al.Norepinephrine up-regulates the expression of vascular endothelial growth factor,matrix metalloproteinase ( MMP) -2, and MMP-9 in nasopharyngeal carcinoma tumor cells[J]. Cancer Res, 2006, 66(21) : 10357-10364.
[7]	Gupte A, Mumper RJ.Elevated copper and oxidative stress in cancer cells as a target for cancer treatment[J]. Cancer Treat Rev, 2009, 35(1) :32-46.
[8]	Dejeans N, Glorieux C, Guenin S, et al.Overexpression of GRP94 in breast cancer cells resistant to oxidative stress promotes high levels of cancer cell proliferation and migration: Implications for tumor recurrence[J]. Free Radic Biol Med, 2012, 52(6):993-1002.
[9]	Masur K, Niggemann B, Zanker KS,et al.Norepinephrine-induced migration of SW 480 colon carcinoma cells is inhibited by beta-blockers[J]. Cancer Res, 2001,61(7) :2866-2869.
[10]	Zhang D, Ma QY, Hu HT, et al.β2-adrenergic antagonists suppress pancreatic cancer cell invasion by inhibiting CREB, NF-κB and AP-1[J]. Cancer Biol Ther, 2010, 10(1):19-29.
[11]	Sood AK, Bhatty R, Kamat AA,et al.Stress hormone-mediated invasion of ovarian cancer cells[J]. Clin Cancer Res, 2006, 12(2) :369-375.
[12]	Lara HE, Dorfman M, Venegas M, et al.Changes in sympathetic nerve activity of the mam-malian ovary during a normal estrous cycle and in polycystic ovary syndrome: Studies on norepinephrine release[J]. Microsc Res Tech , 2002, 59(6) :495-502.
[13]	何振, 张澍田.β-肾上腺素能受体在肿瘤发生发展中的作用[J]. 国际外科学杂志, 2012, 39(7): 475-478.
[14]	魏小勇, 黎才海, 饶荣生. p38MAPK信号通路与肿瘤的关系[J]. 实用癌症杂志, 2009, 24(1) : 101-103.

[1]	YOU Rongli, HUANG Yurong, MAORui, HAI Lina, WANG Yingli, WANG Yan. Mechanism of compound kushen injection in the treatment of lung cancer based on serum metabolomics and network pharmacology [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(9): 988-999.
[2]	FAN Jie, JIN Yongming, JIANG Xiaolong, JIANG Guohua. Molecular mechanism of lncRNA HOTAIR regulating miR-206 on the proliferation and apoptosis of rheumatoid arthritis synovial cells [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(7): 736-742.
[3]	HOU Qiong, LIU Fei, CHEN Chuanrong. Immunotherapy combined with anti-angiogenic drugs and chemotherapy in negative driver gene and advanced non-small cell lung cancer [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(7): 775-779.
[4]	FU Ting, LI Fang, PAN Dayan, XIA Yuanyuan, ZHANG Qiuyuan. miR-718 from exosomes of lung cancer cells induces angiogenesis by targeting PTEN [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(6): 624-632.
[5]	WANG Juan, LIU Weiying, YE Yucai, FU Wenli, ZHANG Sha, LI Leping. Research progress of norepinephrine reuptake inhibitor combined with antimuscarinic in the treatment of obstructive sleep apnea [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(6): 714-720.
[6]	WANG Yan, ZHANG Jinhui, ZHAO Yongchen, LIU Hongxiang, LIU Yawei, MIAO Huanhuan, YANG Xincai. Sequoiaflavone inhibits stem cell properties such as proliferation and invasion of gastric cancer cells by down-regulating PI3K/AKT signaling pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(5): 508-513.
[7]	LI Fei, DING Huiqin, CHEN Mengjing. Isonlosinine induces autophagy of PC9 cells by ERK signaling pathway in lung cancer [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(11): 1235-1240.
[8]	LIU Jiajun, QUE Zujun, TIAN Jianhui. Comparative study on the ability of active ingredients in Jinfukang to decrease neutrophils recruiting by lung cancer circulating tumor cells [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(1): 1-9.
[9]	SU Yang, ZHOU Feng, DING Jinlei. Effects of esketamine combined with sufentanil on postoperative analgesia and emotion after thoracoscopic radical resection of lung cancer [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(1): 59-65.
[10]	HUANG Xiaoming, DU Ye, LIN Shaoming, SHEN Guanle. Exploratory study of the influence of respiratory microbiology on the efficacy of PD-1 inhibitors monotherapy for patients with advanced non-small cell lung cancer [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(1): 66-74.
[11]	ZHANG Kexin, JIA Wenjing, CUI Jiawen, AO Luyao, ZHOU Fang, WANG Guangji, LIU Jiali. Advances in the research and clinical application of the third generation EGFR TKIs in the treatment of non-small cell lung cancer [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(9): 1016-1030.
[12]	DING Jiamin, WANG Youdi, ZHAO Xiali, JIANG Genfeng, CHEN Siwen, HONG Chengcheng, LI Shuqin, HU Weihua. Study on the treatment of thin endometrium with growth hormone and vitamin E [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(8): 857-862.
[13]	WANG Lin, LI Chongyang, LI Shuaihu, GAO Shenghan, XU Tian, LI Fei. Research progress on pathogenic fusion gene in lung cancer [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(8): 877-885.
[14]	SUI Yuxia, ZHUANG Jie, ZHUANG Min, HUANG Gui. Delicaflavone inhibits the invasion and migration of gefitinib-resistant lung cancer PC-9/GR cells by regulating epithelial-mesenchymal transition via PI3K/Akt/mTOR pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(6): 614-621.
[15]	WANG Xinli, XU Xiaqing, FANG Hanbing, GUO Yuzhong. Study of TPA on enhancing the anti-tumor effects of cisplatin and reducing its renal toxicity [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(5): 535-543.