Abstract: AIM: To investigate the effect of poria drug serum on the proliferation of the side population (SP) of gastric cancer cell line SGC-7901. METHODS: The making of poria rabbit serum drug; Sorting the gastric cancer cell line SGC-7901 SP cells by flow cytometry technique; Observe the effect of poria drug serum on the proliferation of the gastric cancer cell line SGC-7901 SP cells by CCK-8; Observe the effect of potia drug serum on the cell cycle of the gastric cancer cell line SGC-7901 SP cells by flow cytometry technique. RESULTS: The percentage of side group in gastric cancer cell line SGC-7901 was 4.2%±0.6%; Gastric cancer cell line SGC-7901 SP cells proliferation ability was significantly inhibited by poria drug serum; The cell cycle of SP cells was blocked in G₀, G₁ phase while the S and G₂/M phase cells were on the decrease after the intervention of poria drug serum. At the same time, longer duration of intervention, the trend was more obvious, so the experimental results of cell proliferation was verified. CONCLUSION: SP cells can be successfully sorted out of gastric cancer SGC-7901 cell lines. With poria drug serum on the change of cell cycle, the proliferation of the gastric cancer cell line SGC-7901 SP cells can be suppressed.

Key words: gastric cancer, side population, poria, proliferation, cell cycle