Abstract: 【Objective】 To investigate the potential roles of tumor necrosis factor-α(TNF-α) and it's solube receptors(sTNF-R1 and sTNF-R2) in the pathogenesis of asthmatic inflammation, the concentrations of TNF-α, sTNF-R1 and sTNF-R2 proteins were determined. 【Methods】 All the patients were obtained including 46 cases of patients with bronchial asthma exacerbation, 31 cases of patients with bronchial asthma paracmasia, and were compared to 22 cases of individuals. Concentrations of TNF-α proteins was detected by enzyme linked immunosorbent assay methods(ELISA). And the concentrations of sTNF-R1 and sTNF-R2 proteins were also determined by ELISA. 【Results】 The concentrations of TNF-α, sTNF-R1 and sTNF-R2 proteins were significantly higher in asthma exacerbation group[(3.19±1.68),(4.69±2.30),(14.32±6.19) ng/mL respectively] than those in control group [(1.96±0.86)、(2.07±0.86)、(4.75±1.68) ng/mL respectively](all P<0.01). Data also showed that the concentrations of sTNF-R1 and sTNF-R2 proteins were markedly lower in bronchial asthma paracmasia group [(2.59±1.21)、(11.57±4.78) ng/mL respectively] than those in asthma exacerbation group(P＜0.01 or ＜0.05, respectively). While no significance were observed of TNF-αexpressions between the patients with asthma paracmasia group(2.75±1.31) ng/mL and in acute phase(P>0.05). When compared to control group, the concentrations of TNF-α and sTNF-R2 proteins in asthma paracmasia group were significantly higher, but there were no significance of sTNF-R1 proteins between them. Furthermore, levels of sTNF-R1 was highly correlated to sTNF-R2(n=99,r=0.239,P＜0.05), but there were no strong association neither between levels of sTNF-R1 and TNF-α, nor between levels of sTNF-R2 and TNF-α(all P>0.05). 【Conclusions】 Levels of TNF-α, sTNF-R1 and sTNF-R2 are elevated in bronchial asthma which could be applied as an effective targets of patient's condition judgment. Our study indicated TNF-α, sTNF-R1 and sTNF-R2 expressions may involved in the pathogenesis of bronchial asthma inflammation.

Key words: bronchial asthma, tumor necrosis factor, receptor, enzyme linked immunosorbent assay

摘要： 【目的】 观察肿瘤坏死因子-α(TNF-α)及其可能性受体(sTNF-R1和sTNF-R2)在支气管哮喘患者中的表达,探讨它们在支气管哮喘炎症机制中的作用。 【方法】 采用ELISA法检测哮喘急性发作期和哮喘缓解期患儿及健康对照组儿童血清TNF-α、sTNF-R1和sTNF-R2的蛋白浓度。 【结果】 哮喘急性发作期组血清TNF-α、sTNF-R1 和sTNF-R2的蛋白浓度[分别为(3.19±1.68)、(4.69±2.30)、(14.32±6.19)] ng/mL显著高于对照组[(1.96±0.86)、(2.07±0.86)、(4.75±1.68)] ng/mL (P均＜0.01);哮喘缓解期组sTNF-R1 和sTNF-R2的蛋白浓度[分别为(2.59±1.21)、(11.57±4.78)] ng/mL显著低于哮喘急性发作期组(分别为P＜0.01和＜0.05),而TNF-α的蛋白浓度(2.75±1.31) ng/mL与哮喘急性发作期组相比差异无统计学意义(P>0.05);哮喘缓解期组TNF-α和sTNF-R2的蛋白浓度显著高于对照组(P均＜0.01),而sTNF-R1的蛋白浓度与对照组相比差异无统计学意义。sTNF-R1与sTNF-R2的表达水平呈显著正相关(n=99,r =0.239,P＜0.05),但sTNF-R1和sTNF-R2的表达水平分别与TNF-α之间无显著相关性(P>0.05)。 【结论】 TNF-α、sTNFR1和sTNFR2可能参与了哮喘的炎症发病过程,哮喘患者血清TNF-α、sTNF-R1和sTNF-R2水平增高可能被作为病情活动的指标之一。

关键词: 哮喘, 肿瘤坏死因子, 受体, 酶联免疫吸附试验