Effects of total glucosides of paeong on improving insulin sensitivity, lowering blood pressure and enhancing antioxidation in metabolic syndrome-hypertensive rats

Abstract

Abstract: AIM: To investigate the antihypertensive effects and effective mechanisms of total glucosides of paeong(TGP for short)on insulin resistance, hypertension,and the changes of oxidative stress in metabolic syndromic(MS)-hypertensive(Ht)rats. METHODS: The SD rats were fed with a high fat(25%)-sucrose (10%)-enriched diet qd for 8 weeks. When the rats were exposed hypertension and impaired glucose tolerance (IGT),and the rats were randomly divided into normal control group, metformin (Met) group 200 mg/kg, and TGP 150, 50 mg/kg, solvent or the drugs were adiministered by intragastric administration (i.g.) qd for 4 weeks. The blood pressure (BP), the contents of fasting blood glucose (FBG), 2-hours blood glucose after oral glucose tolerance test (2 h BG-OGTT), and fasting insulin (Fins) were determined, as well as the insulin sensitivity index (ISI) and insulin resistance index (HOMA-IRI) were calculated; the contents of free fatty acids (FFA), TNF-α, endothelin (ET-1), renin-angiotensinⅡ, NO,malonaldehyde (MDA) and activities of nitric oxide synthase (NOS) and superoxide dismutase (SOD) activity were determined after treatment. RESULTS: Compared with normal control group,the BP and the contents of FBG,OGTT-2 h BG,Fins were incresaed in MS-Ht rats,and ISI was weaken, HOMA-IRI was incresaed. the contents of FFA,TNF-α,ET-1,renin-Ang Ⅱ,MDA were incresaed;the content of NO,the activities of NOS and SOD were decreased(P<0.05 or P<0.01). The ISI was incresaed,the HOMA-IRI was decreased,retrieve hyperinsulinemia(P<0.05 or P<0.01), the contents of FFA,TNF-α,ET-1,renin-Ang Ⅱ,MDA were decreased,the activities of NO,NOS,SOD were elevated in Met group and TGP 150 mg/kg group. Compared with TGP 150 mg/kg group,the contents of FBG,OGTT-2 h BG were decreased in Met group,there was no significant difference. CONCLUSION: TGP and Met are able to enhance the insulin sensitivity, retrieve hyperinsulinemia,Antagonism of ET-1, renin-Ang Ⅱ system and oxidative stress response,augment the function of NO, NOS, degrade the BP;TGP does not have a strong predominance against hyperglycaemia, TGP differs from Met. The effects of TGP show dose-effect relationship.

Key words: Total glucosides of paeong, Metabolic syndrome, Hypertension, Insulin resistance, Rennin-angiotensin system, TNF-α, Vasoactive substance, Rat

CLC Number:

R965.2

FENG Rui-er, ZHENG Lin-ying, LV Jun-hua, PAN Jing-qiang, XIAO Liu-ying. Effects of total glucosides of paeong on improving insulin sensitivity, lowering blood pressure and enhancing antioxidation in metabolic syndrome-hypertensive rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(2): 154-159.

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