Effects of sulforaphane-induced Akt/p70S6K signal transduction pathway on apoptosis of human nasopharyngeal cancer cells CNE-2

doi:10.12092/j.issn.1009-2501.2019.07.010

Abstract

Abstract:

AIM: To study the effect of sulforaphane on the proliferation and apoptosis of CNE-2 cells, and to investigate the role of sulforaphane in the Akt/p70S6K signaling pathway. METHODS: CCK-8 test was used to detect cellular growth of CNE-2. Flow cytometry was carried out to observe apoptosis and cell cycle status. Expression levels of several cell cycle associated proteins and Akt/p70S6K pathway related proteins were determined by Western blot.RESULTS:Cell proliferation inhibitory rates and apoptotic rates of CNE-2 cells in various doses of sulforaphane groups were significantly increased as compared with control group with a dose-dependent manner (P<0.05). Flow cytometry results showed that sulforaphane arrestted NPC cells at the S-phases and G2/M-phases, and the expression levels of Cyclin A, Cyclin B, Cyclin D, Cyclin E and CDK/p34 were greatly decreased in sulforaphane group as compared with control group (P<0.05). Moreover, the expression levels of p-Akt and p70S6K in sulforaphane group were also decreased as compared with control group (P<0.05). However, the expression levels of these proteins above in sulforaphane+ IGF-I group unchanged as compared with control group (P>0.05). CONCLUSION: Sulforaphane exerts anti-proliferative and pro-apoptotic effects on CNE-2 cells through interfering with the Akt/p70S6K signaling pathways.

Key words: sulforaphane, human nasopharyngeal cancer cells, proliferation, apoptosis, Akt/p70S6K signaling pathways

CLC Number:

R739.6
R966

ZHAO Li, HUA Xia, TAN Ye, HU Chenglian. Effects of sulforaphane-induced Akt/p70S6K signal transduction pathway on apoptosis of human nasopharyngeal cancer cells CNE-2[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(7): 786-791.

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