Effects of droperidol on the enhancement of persistent sodium currents induced by in vitro ischemia-like condition in isolated rat CA1 paramidal neurons

Abstract

Abstract: AIM: To study the effects of droperidol on the enhancement of persistent sodium currents induced by in vitro ischemia-like condition in isolated rat CA1 paramidal neurons. METHODS: Whole-cell patch-clamp recordings were made from enzymatically isolated rat CA1 hippocampal paramidal neurons.Ischemia was induced by oxygen and glucose deprivation. RESULTS: All of 3, 10, and 30 μmol·L ^-1of droperidol significantly inhibited the enhancement of persistent sodium currents induced by ischemia, but the different concentrations did not show significant difference. CONCLUSION: Droperidol in clinical concentration can inhibit the enhancement of persistent sodium current induced by ischemia.

Key words: pharmacodynamics, droperidol, persistent sodium current, patch-clamp, hippocampal, ischemia

CLC Number:

R973.3
R965

JIAO Zhi-Hua, ZHUANG Xin-Liang, WANG Shi-Lei. Effects of droperidol on the enhancement of persistent sodium currents induced by in vitro ischemia-like condition in isolated rat CA1 paramidal neurons[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2003, 8(1): 48-51.

References

[1] Friedman JE, Haddad GG.Anoxia induced an increase in intracellular sodium in rat cortical neurons in virto[J].Brain Res, 1994;663:57-64
[2] Hammarstrom AK, Gage PW.Inhibition of oxidative metabolism increases persistent sodium current in rat CA1 hippocampal neurons[J].J Physiol, 1998;510:735-41
[3] 郑斯聚. 镇静安定药[A].见:刘俊杰, 赵俊, 主编.现代麻醉学[M].第2 版.北京:人民卫生出版社, 1996:334
[4] Buckley NA, Sanders P.Cadiaovascular adverse effects of antipsychotic drugs[J].Drug Safe, 2000;23:215-28
[5] Olschewski A, Brau ME, Hempelmann G, et al.Differential block of fast and slow inactivating tetrodotoxin-sensitive sodium channels by droperidol in spinal dorsal horn neurons[J].Anesthesiology, 2000;92:1667-76
[6] 张宗明, 吴才宏, 阳冬梅, 等.大白鼠海马CA1 区锥体细胞的急性分离及其电生理学特性[J].北京医科大学学报, 1997;29:5-7
[7] Mitani A, Yanase H, Sakai K, et al.Origin of intracellular Ca2 + elevation induced by in vitro ischemia-like condition in hippocampal slices[J].Brain Res, 1993;601:103-10
[8] Crill WE.Persistent sodium current in mammalian central neurons[J].Annu Rev Physiol, 1996;58:349-62
[9] Alzheimer C, Schwindt PC, Crill WE.Modal gating of Na + channels as a mechanism of persistent Na+ current in pyramidal neurons from rat and cat sensorimotor cortex[J].J Neurosci, 1993, 13:660-73
[10] Chidekel AS, Friedman JE, Haddad GG.Anoxia-induced neuronal injury:role of Na+ entry and Na+-dependent transport [J].Exp Neurol, 1997;146:403-13
[11] Fung ML.Role of voltage-gated Na+ channels in hypoxia-induced neuronal injuries [J].Clin Exp Pharmacol Physiol, 2000;8:569-74
[12] Raley-Susman KM, Kass IS, Cotfrell JE, et al.Sodium influx blocked and hypoxic panage to CA1 pyramidal neurons in rat hippocampal slices[J].J Neurophysiol, 2001;86:2715-26
[13] Fried E, Amorim P, Chambers G, et al.The importance of sodium for anoxic transmission damage in rat hippocampal slices:mechanisms of protection by lidocaine [J].J Physiol, 1995;489:557-65
[14] Fischler M, Bonnet F, Trang H, et al.The pharmacokinetics of droperidol in anesthetized patients [J].Anesthesiology, 1986;64:486-9

[1]	WU Yujie, ZHAO Chengcheng, XI Qing. Evaluation of tegacycline regimens in treatment of gram-negative bacterial infections with Monte Carlo simulation [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(9): 1027-1033.
[2]	HOU Xiaoyu, LENG Yufang, CAO Xuefen, LV Xingjiao, HAN Xiaoxia, Janvier NIBARUTA, LIU Yongqiang. Network analysis and experimental verification of Schisandrin B reduces intestinal ischemia reperfusion injury [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(2): 147-154.
[3]	CAO Xuefen, LENG Yufang, HAN Xiaoxia, HOU Xiaoyu, LYU Xingjiao, Janvier NIBARUTA. Tetramethylpyrazine protected against intestinal ischemia-reperfusion injury induced pyroptosis by inhibiting NLRP3 inflammasome activation [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(11): 1201-1208.
[4]	ZHANG Jingyu, WANG Yihan, LI Jun, JIN Ping, SHEN Xiping, WANG Yingbin, LIU Jieting. Involvement of autophagy in iron ion regulation promotes ferroptosis in cells undergoing intestinal ischemia-reperfusion injury [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(1): 36-41.
[5]	ZHANG Wei, WANG Yingbin, CAO Lu, LIU Yan, ZHANG Li, LIU Jieting. Research progress of autophagy in intestinal ischemia-reperfusion injury [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(9): 1061-1066.
[6]	HUA Haiyan, YAN Jie, QIN Yufen. Experimental study of schisandrin B attenuates acute myocardial ischemia injury in rats by inhibiting cardiomyocyte apoptosis [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(8): 848-856.
[7]	HAN Xiaoxia, LENG Yufang, LV Xingjiao, HOU Xiaoyu, CAO Xuefen, Janvier NIBARUTA. Research progress on the role and mechanism of irisin in organ ischemia/reperfusion injury [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(8): 886-891.
[8]	GU Yifei, CHU Nannan, HUANG Kai, QUE Linling, ZHANG Jisheng, XIANG Xuemei, HE Qing. Application and progress of pharmacodynamics study in bioequivalence evaluation of orally inhaled drug products [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(7): 822-833.
[9]	LV Xingjiao, LENG Yufang, HAN Xiaoxia, HOU Xiaoyu, CAO Xuefen, Janvier NIBARUTA, LIU Yongqiang . Research progress of short-chain fatty acids in alleviating organ ischemia-reperfusion injury [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(2): 219-226.
[10]	ZHANG Jinhua, LIU Maobai, CAI Mingzhi, ZHENG Yingli, LAO Haiyan, XIANG Qian, DU Liping, ZHU Zhu, DONG Jing, ZUO Xiaocong, LI Xingang, SHANG Dewei, CHEN Bing, YE Yanrong, WANG Yuzhu, GAO Jianjun, ZHANG Jian, CHEN Wansheng, XIE Haitang, JIAO Zheng. Model informed precision dosing of warfarin: China expert consensus report (2022 version) [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(11): 1201-1212.
[11]	TAN Fenglang, REN Hongli, WANG Hongfei. Effect of Phellodendri amurense extract on hippocampal nerve cells induced by Aβ_1-42 [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(11): 1213-1220.
[12]	WU Lin, HAO Yingxiang, ZHANG Lei, ZHANG Tianxue, GAO Mingjing, LENG Yufang. Paricalcitol attenuates intestinal ischemia-reperfusion injury in mice through HMGB1/TLR4/NF-κB signaling pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(1): 1-7.
[13]	XU Liying, ZHANG Gaosong, HUANG Xiaomin, HUANG Jihan, WANG Shuhua. Clinical efficacy and modeling evaluation of He-wei-zhi-xie capsules in treating diarrhea patients [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(1): 47-55.
[14]	ZHANG Jianmin, LENG Yufang, LIU Xin, REN Yixing, SHI Yajing, CHEN Feng. Research progress on the mechanism of miRNA in intestinal ischemia-reperfusion injury [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2021, 26(3): 332-340.
[15]	YUN Qiang, DONG Xuejia, WANG Mengjiao, LIU Yahong, WANG Zhiguang, JIANG Mingfang. Rosuvastatin regulates cerebral I/R damage to neurons by UCP2-SIRT3 signal [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2021, 26(2): 144-153.