Abstract: AIM: To observe protective effects of ethyl ferulate (EF) on injured vascular endothelial cells (VECs) by hydrogen peroxide (H₂O₂).METHODS: Experimental groups were set as control group, H₂O₂group, ethyl ferulate group and ferulic acid (AF) group.The proliferation of vascular endothelial cells was assessed by the methyl thiazolyl tetrazolium (MTT) assay.Malonaldehyde (MDA) and lactate dehyolrogenase (LDH) were determined to evaluate lipid peroxidation of VECs by the spectro photometric assay.RESULTS: Ethyl ferulate and ferulic acid at concentrations of 2, 4, 8, and 16 nmol·L^-1 could protect vascular endothelial cells from the injure by H₂O₂ and reduce LDH and MDA production.CONCLUSION: Ethyl ferulate exerts the protective effects on injured vascular endothelial cells by H2O2.The activity of EF is higher than that of AF.Its mechanism may be related to antioxidant effect of lipids peroxidation.

Key words: ethyl ferulate, vascular endothelial cells, hydrogen peroxide, MTT