Abstract: AIM: Artificial bloody cerebrospinal fluid(BCSF) was used as pathogeny on human umbilical vein endothelial cells (HUVECs) to mimic SAH status in vivo.We tried to explore expression of ICAM-1 in HUVECs and the effect of ecdysterone (EDS).METHODS: HUVECs were used for study.BCSF was added into the culture with or without ecdysterone (200 mg·L^-1) for 24 h,and then immunocytochemistry and RT-PCR were used to observe ICAM-1 expression in HUVECs.RESULTS: Following BCSF stimulation, both of immunocytochemistry and expression of ICAM-1 mRNA increased in HUVECs.EDS could reduce the expression of ICAM-1 in HUVECs following BCSF stimulation.CONCLUSION: ICAM-1expression in HUVECs could be activated directly by BCSF and reduced by EDS.EDS is possible to be a valid candidate medicine in therapy of CVS.

Key words: cerebral vasospasm, subarachnoid hemorrhage, endothelial cells, intracellular adhesion molecule-l, ecdysterone