Abstract: AIM: To observe the preventive effects of Ligustrazine (Lig) on bone loss induced by Cyclophsphamide (CY) in male rats. METHODS: Thirty 3-monthold Sprague-Dawley male rats were randomly divided into 3 groups. Rats in control group were treated by daily oral gavage with vehicle. Rats in other two groups were treated by either daily oral gavage with CY at 4.5mg·kg^-1·d ^-1, or CY at 4.5 mg·kg^-1·d ^-1 and Lig at 50 mg·kg^-1·d^-1 for 15 days. At the endpoint the right femoral was collected to determine bone weight, contents of calcium (Ca) and Hydroxyproline(Hyp). The number of leukocyte, the weight of thymus and spleen were determined, respectively. RESUITS: The contents of Ca and Hyp of humerus, number of leukocyte, weights of thymus and spleen in CY group were decreased when compared with those in control group, while those in Lig group were greater than those in CY group. CONCLUSION: Lig could prevent bone loss induced by CY in male rats.

Key words: Cyclophsphamide, Ligustrazine, rat