Effect of Curcumin on proliferation inhibition induced by Urotensin-II in human lens epithelial cell and its mechanism of signal transduction

Abstract

Abstract: AIM: To study the effect of Curcumin (Cur)on proliferation inhibition induced by urotensin-II in human lens epithelial cell (HLEC)and its signal transduction mechanism of [Ca²⁺] i, cAMP and cGMP. METHODS: The cultured HLEC were incubated with different concentrations of Cur and the HLEC activities were detected viaMTT assay.The proliferative cell nuclear antigen (PCNA)of HLEC were detected via flow cytometer(FCM).The concentration of intracellular free calcium ([Ca²⁺] i)of HLEC were determined by spectrofluoremeter.The intracellular contents of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP)of HLEC were detected by radioimmunoassay. RESULTS: The activity, expression of PCNA and the cGMP concentration of HLEC in U-II group were higher than those in control group, but they were significantly decreased in Cur group (P <0.01).Compared with control group, the [Ca²⁺] i concentration of HLEC in U-II group was increased, and the cAMP concentration in U-II group was decreased (P < 0.01).Compared with U-II group, the concentrations of cAMP and [Ca²⁺] i were increased (P <0.01). CONCLUSION: Cur can inhibit the proliferation of HLEC induced by urotensin- II.The signal transduction of [Ca²⁺], cAMP-PKA and cGMP-PKG may play an important role in it.Cur may become an effective drug of preventing and treating after-cataract.

Key words: Urotensin II, Curcumin, lens epithelial cell, proliferation, signal transduction, after-cataract

CLC Number:

R965.2

QI Ming-xin, HUANG Xiu-rong, HU Jun, YANG Li-ying. Effect of Curcumin on proliferation inhibition induced by Urotensin-II in human lens epithelial cell and its mechanism of signal transduction[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(10): 1085-1090.

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