Expression of matrix metalloprotease2/9 and tissue inhibitor of metalloproteinase- 2 in the liver tissue of rats with vibrio vulnificus sepsis and the effects of antibacterial agents on these genes

Abstract

Abstract: AIM: To observe the gene expression of matrix metalloprotease 2/9 (MMP-2/9) and tissue inhibitor of metalloproteinase-2 (TIMP-2) in the liver tissues of rats with vibrio vulnificus sepsis and the effects of cefoperazone godium in combination of levofloxacin on the expression of gene MMP2 9 and TIMP-2.METHODS: 110 depuratory grade Sprague-Dawley male rats were randomly divided into three groups, normal control group (CN group, n=10) , vibrio vulnificus sepsis model groups (VV group, n=50) and vibrio vulnificus sepsis model treated with cefoperazone godium in combination of levof loxacin (AA groups, n=50).The ethological changes were observed in all group rats.The expression of MMP2 9 and TIMP2 mRNA were detected by reverse transcriptive polymerase chain reaction (RT-PCR).RESULTS: The levels of gene expression of MMP2 9 were markedly up-regulated and TIMP-2 were down-regulated at early time in all VV groups (via CN group, P<0.05).However, the levels of gene expression of MMP2 9 were down-regulated and TIMP-2 were up-regulated after interruption by antibiotics (via VV group, P<0.05).CONCLUSION: The up-regulation of MMP-2 9 and down-regulation of TIMP-2 are associated with the pathophysiological processes of vibrio vulnificus sepsis, the treatment with cefoperazone sodium in combination with levofloxacin may down-regulate the expressions of MMP-2 9 and up-regulate TIMP-2 gene expression in the liver tissue of rats with vibrio vulnificus sepsis, and relieve the clinical symptoms.

Key words: vibrio vulnificus, sepsis, metalloproteinase, antibiotics

CLC Number:

R965.2

LI Jing-rong, XIONG Shu-dao, LIANG Huan, LU Zhong-qiu. Expression of matrix metalloprotease2/9 and tissue inhibitor of metalloproteinase- 2 in the liver tissue of rats with vibrio vulnificus sepsis and the effects of antibacterial agents on these genes[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(4): 410-415.

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